In vitro 3D colective angiogenic response under orchestrated multiple chemical gradients

Jessie S. Jeon, Yoojin Shin, Jeonghun Nam, Seungha Lee, Gi Seok Jung, Sehyun Shin, Sang Hoon Lee, Roger D. Kamm, Seok Chung

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Angiogenesis is a process requiring a coordinated guidance from multiple factors. We examined the effects of angiogenic endothelial cell sprouting under the gradient of soluble factors, VEGF and ANG-1. This resulted in a different cellular morphology than the condition with single factors; the presence of VEGF gradient induced a greater number of tip cells, and addition of ANG-1 to the VEGF gradient stabilized collectively migrating cells. Interestingly, as shown by the average filopodial angle measured, tip cell was affected by VEGF only when ANG-1 gradient was not present, possibly because the tip cells attached to stalk cells have reduced freedom.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages944-946
Number of pages3
Publication statusPublished - 2010 Dec 1
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

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Keywords

  • Angiogenesis
  • Cell migration
  • Cell-on-a-chip

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Jeon, J. S., Shin, Y., Nam, J., Lee, S., Jung, G. S., Shin, S., Lee, S. H., Kamm, R. D., & Chung, S. (2010). In vitro 3D colective angiogenic response under orchestrated multiple chemical gradients. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (pp. 944-946). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).