Abstract
Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.
| Original language | English |
|---|---|
| Pages (from-to) | 981-994 |
| Number of pages | 14 |
| Journal | Plant Molecular Biology |
| Volume | 59 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - 2005 Dec 1 |
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Keywords
- CMV 1a protein
- Nicotiana tabacum
- Thaumatin-like protein (TLP)
- Yeast three-hybrid
- Yeast two-hybrid
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
Cite this
In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins. / Kim, Min Jung; Ham, Byung Kook; Kim, Hwa Ran; Lee, In Ju; Kim, Young Jin; Ryu, Ki Hyun; Park, Young In; Paek, Kyung Hee.
In: Plant Molecular Biology, Vol. 59, No. 6, 01.12.2005, p. 981-994.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins
AU - Kim, Min Jung
AU - Ham, Byung Kook
AU - Kim, Hwa Ran
AU - Lee, In Ju
AU - Kim, Young Jin
AU - Ryu, Ki Hyun
AU - Park, Young In
AU - Paek, Kyung Hee
PY - 2005/12/1
Y1 - 2005/12/1
N2 - Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.
AB - Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.
KW - CMV 1a protein
KW - Nicotiana tabacum
KW - Thaumatin-like protein (TLP)
KW - Yeast three-hybrid
KW - Yeast two-hybrid
UR - http://www.scopus.com/inward/record.url?scp=27944472736&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=27944472736&partnerID=8YFLogxK
U2 - 10.1007/s11103-005-2619-y
DO - 10.1007/s11103-005-2619-y
M3 - Article
C2 - 16307370
AN - SCOPUS:27944472736
VL - 59
SP - 981
EP - 994
JO - Plant Molecular Biology
JF - Plant Molecular Biology
SN - 0167-4412
IS - 6
ER -