In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins

Min Jung Kim, Byung Kook Ham, Hwa Ran Kim, In Ju Lee, Young Jin Kim, Ki Hyun Ryu, Young In Park, Kyung Hee Paek

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

Original languageEnglish
Pages (from-to)981-994
Number of pages14
JournalPlant Molecular Biology
Volume59
Issue number6
DOIs
Publication statusPublished - 2005 Dec 1

Fingerprint

Cucumovirus
Cucumber mosaic virus
Plantae
Viruses
Nicotiana tabacum
Tobacco
Proteins
proteins
Genes
Yeast
yeasts
In Vitro Techniques
Polymerase chain reaction
two hybrid system techniques
Two-Hybrid System Techniques
Capsid Proteins
Transcription
genes
Hybrid systems
Escherichia coli

Keywords

  • CMV 1a protein
  • Nicotiana tabacum
  • Thaumatin-like protein (TLP)
  • Yeast three-hybrid
  • Yeast two-hybrid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins. / Kim, Min Jung; Ham, Byung Kook; Kim, Hwa Ran; Lee, In Ju; Kim, Young Jin; Ryu, Ki Hyun; Park, Young In; Paek, Kyung Hee.

In: Plant Molecular Biology, Vol. 59, No. 6, 01.12.2005, p. 981-994.

Research output: Contribution to journalArticle

Kim, Min Jung ; Ham, Byung Kook ; Kim, Hwa Ran ; Lee, In Ju ; Kim, Young Jin ; Ryu, Ki Hyun ; Park, Young In ; Paek, Kyung Hee. / In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins. In: Plant Molecular Biology. 2005 ; Vol. 59, No. 6. pp. 981-994.
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