Abstract
Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.
Original language | English |
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Pages (from-to) | 981-994 |
Number of pages | 14 |
Journal | Plant Molecular Biology |
Volume | 59 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2005 Dec |
Keywords
- CMV 1a protein
- Nicotiana tabacum
- Thaumatin-like protein (TLP)
- Yeast three-hybrid
- Yeast two-hybrid
ASJC Scopus subject areas
- Agronomy and Crop Science
- Genetics
- Plant Science