In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins

Min Jung Kim; Byung Kook Ham; Hwa Ran Kim; In Ju Lee; Young Jin Kim; Ki Hyun Ryu; Young In Park; Kyung Hee Paek

doi:10.1007/s11103-005-2619-y

In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins

Min Jung Kim, Byung Kook Ham, Hwa Ran Kim, In Ju Lee, Young Jin Kim, Ki Hyun Ryu, Young In Park, Kyung Hee Paek

Division of Life Sciences

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

Original language	English
Pages (from-to)	981-994
Number of pages	14
Journal	Plant Molecular Biology
Volume	59
Issue number	6
DOIs	https://doi.org/10.1007/s11103-005-2619-y
Publication status	Published - 2005 Dec

Keywords

CMV 1a protein
Nicotiana tabacum
Thaumatin-like protein (TLP)
Yeast three-hybrid
Yeast two-hybrid

ASJC Scopus subject areas

Agronomy and Crop Science
Genetics
Plant Science

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In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins. / Kim, Min Jung; Ham, Byung Kook; Kim, Hwa Ran; Lee, In Ju; Kim, Young Jin; Ryu, Ki Hyun; Park, Young In; Paek, Kyung Hee.

In: Plant Molecular Biology, Vol. 59, No. 6, 12.2005, p. 981-994.

Research output: Contribution to journal › Article › peer-review

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title = "In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins",

abstract = "Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.",

keywords = "CMV 1a protein, Nicotiana tabacum, Thaumatin-like protein (TLP), Yeast three-hybrid, Yeast two-hybrid",

author = "Kim, {Min Jung} and Ham, {Byung Kook} and Kim, {Hwa Ran} and Lee, {In Ju} and Kim, {Young Jin} and Ryu, {Ki Hyun} and Park, {Young In} and Paek, {Kyung Hee}",

note = "Funding Information: This work was supported by grants from the Plant Signaling Network Research Center (Grant #R11-2003-008-02001-0) and the Agriculture R&D Promotion Center (Grant #203058-3) to K.-H. Paek. B.-K. Ham was supported by a postdoctoral fellowship from the KOSEF, and the scholarships of the graduate students were provided by the BK21 program of Korea.",

year = "2005",

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doi = "10.1007/s11103-005-2619-y",

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volume = "59",

pages = "981--994",

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AU - Kim, Min Jung

AU - Ham, Byung Kook

AU - Kim, Hwa Ran

AU - Lee, In Ju

AU - Kim, Young Jin

AU - Ryu, Ki Hyun

AU - Park, Young In

AU - Paek, Kyung Hee

N1 - Funding Information: This work was supported by grants from the Plant Signaling Network Research Center (Grant #R11-2003-008-02001-0) and the Agriculture R&D Promotion Center (Grant #203058-3) to K.-H. Paek. B.-K. Ham was supported by a postdoctoral fellowship from the KOSEF, and the scholarships of the graduate students were provided by the BK21 program of Korea.

PY - 2005/12

Y1 - 2005/12

N2 - Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

AB - Using a yeast two-hybrid system, we identified a plant cellular factor that interacts with the proteins of the Cucumber mosaic virus (CMV). Initially 14 candidate genes were isolated from Nicotiana tabacum, using a full-length CMV 1a gene as bait. Among the candidate genes, two were encoding thaumatin-like proteins (TLP), and were designated as Nicotiana tabacum thaumatin-like protein 1 (NtTLP1). Consistent with this observation, recombinant GST-NtTLP1 protein, which was expressed and purified in E. coli, bound tightly to CMV 1a in vitro. In planta interaction was also verified via co-immunoprecipitation. Additionally, NtTLP1 specifically interacted with the CMV movement-related proteins, movement protein and coat protein, in yeast. Real-time quantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the expression of NtTLP1 increased as the result of CMV inoculation.

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KW - Yeast two-hybrid

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In vitro and in planta interaction evidence between Nicotiana tabacum thaumatin-like protein 1 (TLP1) and Cucumber mosaic virus proteins

Abstract

Keywords

ASJC Scopus subject areas

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