In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds

Tae Hoon Kim, Young Pil Yun, Young Eun Park, Suk Ha Lee, Woonjae Yong, Joydip Kundu, Jin Woo Jung, Jin Hyung Shim, Dong Woo Cho, Sung Eun Kim, Hae Ryong Song

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The aim of this study was to develop novel polycaprolactone/poly(lactic-co- glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOPA/PCL/PLGA on rat femur had more bone formation than BMP-2/Hep/PCL/PLGA and PCL/PLGA in vivo.

Original languageEnglish
Article number025008
JournalBiomedical Materials (Bristol)
Volume9
Issue number2
DOIs
Publication statusPublished - 2014 Apr 1

Fingerprint

Layered manufacturing
Polycaprolactone
Scaffolds (biology)
Osteogenesis
Scaffolds
Bone
Proteins
Bone and Bones
Acids
Heparin
Dopamine
Osteoblasts
Phosphatases
Femur
Tomography
Alkaline Phosphatase
Milk
polycaprolactone
polylactic acid-polyglycolic acid copolymer
In Vitro Techniques

Keywords

  • bone formation
  • bone morphogenic protein-2 (BMP-2)
  • freeform fabrication (SFF)
  • osteoblast activity
  • poly(lactic-co- glycolic acid) (PLGA)
  • polycaprolactone (PCL)
  • solid

ASJC Scopus subject areas

  • Chemistry (miscellaneous)
  • Business and International Management
  • Mechanics of Materials

Cite this

In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds. / Kim, Tae Hoon; Yun, Young Pil; Park, Young Eun; Lee, Suk Ha; Yong, Woonjae; Kundu, Joydip; Jung, Jin Woo; Shim, Jin Hyung; Cho, Dong Woo; Kim, Sung Eun; Song, Hae Ryong.

In: Biomedical Materials (Bristol), Vol. 9, No. 2, 025008, 01.04.2014.

Research output: Contribution to journalArticle

Kim, Tae Hoon ; Yun, Young Pil ; Park, Young Eun ; Lee, Suk Ha ; Yong, Woonjae ; Kundu, Joydip ; Jung, Jin Woo ; Shim, Jin Hyung ; Cho, Dong Woo ; Kim, Sung Eun ; Song, Hae Ryong. / In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds. In: Biomedical Materials (Bristol). 2014 ; Vol. 9, No. 2.
@article{d0e0c19b216446e2b885bf1776a942e6,
title = "In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds",
abstract = "The aim of this study was to develop novel polycaprolactone/poly(lactic-co- glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOPA/PCL/PLGA on rat femur had more bone formation than BMP-2/Hep/PCL/PLGA and PCL/PLGA in vivo.",
keywords = "bone formation, bone morphogenic protein-2 (BMP-2), freeform fabrication (SFF), osteoblast activity, poly(lactic-co- glycolic acid) (PLGA), polycaprolactone (PCL), solid",
author = "Kim, {Tae Hoon} and Yun, {Young Pil} and Park, {Young Eun} and Lee, {Suk Ha} and Woonjae Yong and Joydip Kundu and Jung, {Jin Woo} and Shim, {Jin Hyung} and Cho, {Dong Woo} and Kim, {Sung Eun} and Song, {Hae Ryong}",
year = "2014",
month = "4",
day = "1",
doi = "10.1088/1748-6041/9/2/025008",
language = "English",
volume = "9",
journal = "Biomedical Materials (Bristol)",
issn = "1748-6041",
publisher = "IOP Publishing Ltd.",
number = "2",

}

TY - JOUR

T1 - In vitro and in vivo evaluation of bone formation using solid freeform fabrication-based bone morphogenic protein-2 releasing PCL/PLGA scaffolds

AU - Kim, Tae Hoon

AU - Yun, Young Pil

AU - Park, Young Eun

AU - Lee, Suk Ha

AU - Yong, Woonjae

AU - Kundu, Joydip

AU - Jung, Jin Woo

AU - Shim, Jin Hyung

AU - Cho, Dong Woo

AU - Kim, Sung Eun

AU - Song, Hae Ryong

PY - 2014/4/1

Y1 - 2014/4/1

N2 - The aim of this study was to develop novel polycaprolactone/poly(lactic-co- glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOPA/PCL/PLGA on rat femur had more bone formation than BMP-2/Hep/PCL/PLGA and PCL/PLGA in vivo.

AB - The aim of this study was to develop novel polycaprolactone/poly(lactic-co- glycolic acid) (PCL/PLGA) scaffolds with a heparin-dopamine (Hep-DOPA) conjugate for controlled release of bone morphogenic protein-2 (BMP-2) to enhance osteoblast activity in vitro and also bone formation in vivo. PCL/PLGA scaffolds were prepared by a solid freeform fabrication method. The PCL/PLGA scaffolds were functionalized with Hep-DOPA and then BMP-2 was sequentially coated onto the Hep-DOPA/PCL/PLGA scaffolds. The characterization and surface elemental composition of all scaffolds were evaluated by scanning electron microscope and x-ray photoelectron spectroscopy. The osteoblast activities on all scaffolds were assessed by cell proliferation, alkaline phosphatase (ALP) activity and calcium deposition in vitro. To demonstrate bone formation in vivo, plain radiograph, micro-computed tomography (micro-CT) evaluation and histological studies were performed after the implantation of all scaffolds on a rat femur defect. Hep-DOPA/PCL/PLGA had more controlled release of BMP-2, which was quantified by enzyme-linked immunosorbent assay, compared with Hep/PCL/PLGA. The in vitro results showed that osteoblast-like cells (MG-63 cells) grown on BMP-2/Hep-DOPA/PCL/PLGA had significantly enhanced ALP activity and calcium deposition compared with those on BMP-2/Hep/PCL/PLGA and PCL/PLGA. In addition, the plain radiograph, micro-CT evaluation and histological studies demonstrated that the implanted BMP-2/Hep-DOPA/PCL/PLGA on rat femur had more bone formation than BMP-2/Hep/PCL/PLGA and PCL/PLGA in vivo.

KW - bone formation

KW - bone morphogenic protein-2 (BMP-2)

KW - freeform fabrication (SFF)

KW - osteoblast activity

KW - poly(lactic-co- glycolic acid) (PLGA)

KW - polycaprolactone (PCL)

KW - solid

UR - http://www.scopus.com/inward/record.url?scp=84896846150&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896846150&partnerID=8YFLogxK

U2 - 10.1088/1748-6041/9/2/025008

DO - 10.1088/1748-6041/9/2/025008

M3 - Article

C2 - 24518200

AN - SCOPUS:84896846150

VL - 9

JO - Biomedical Materials (Bristol)

JF - Biomedical Materials (Bristol)

SN - 1748-6041

IS - 2

M1 - 025008

ER -