In vitro and in vivo IgE-/antigen-mediated mast cell activation

Hae Woong Choi, Soman N. Abraham

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Mast cells (MCs) are major effectors of IgE-mediated allergic reactions because of their unique peripheral location and their powerful capacity to release prestored and de novo-synthesized inflammatory mediators into the circulation upon activation. In view of the growing incidence of allergy worldwide, there is much interest in developing novel strategies to block or temper IgE-mediated MC activation and its pathological consequences. For these studies, standard assays to measure IgE-mediated MC degranulation and mediator release are required. Here, we present detailed procedures to assess in vitro and in vivo MC release of prestored as well as recently synthesized mediators following IgE-/antigen-mediated activation.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages71-80
Number of pages10
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1799
ISSN (Print)1064-3745

Keywords

  • Anaphylaxis
  • Degranulation
  • IgE
  • Mast cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'In vitro and in vivo IgE-/antigen-mediated mast cell activation'. Together they form a unique fingerprint.

Cite this