In vitro anti-adhesive activity of green tea extract against pathogen adhesion

Ji Hye Lee, Jin Sun Shim, Mi Sook Chung, Seung Taik Lim, Kyung Hyun Kim

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract-4 (CSI-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01-0.5 mg/mL. CSI-4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC 50 values (50% inhibition of adhesion) of 0.14-2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI-4 may exert a selective anti-adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria.

Original languageEnglish
Pages (from-to)460-466
Number of pages7
JournalPhytotherapy Research
Volume23
Issue number4
DOIs
Publication statusPublished - 2009 Apr 1

Fingerprint

Propionibacterium acnes
Tea
Adhesives
Camellia sinensis
Bacteria
Uronic Acids
Lactobacillus acidophilus
Cell Line
Staphylococcus epidermidis
Catechin
Hemagglutination
Microbial Sensitivity Tests
Helicobacter pylori
Polysaccharides
Staphylococcus aureus
Carbohydrates
Escherichia coli
Water
In Vitro Techniques
Bifidobacterium bifidum

Keywords

  • Anti-adhesive
  • Green tea
  • Helicobacter pylori
  • Propionibacterium acnes
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Pharmacology
  • Medicine(all)

Cite this

In vitro anti-adhesive activity of green tea extract against pathogen adhesion. / Lee, Ji Hye; Shim, Jin Sun; Chung, Mi Sook; Lim, Seung Taik; Kim, Kyung Hyun.

In: Phytotherapy Research, Vol. 23, No. 4, 01.04.2009, p. 460-466.

Research output: Contribution to journalArticle

Lee, Ji Hye ; Shim, Jin Sun ; Chung, Mi Sook ; Lim, Seung Taik ; Kim, Kyung Hyun. / In vitro anti-adhesive activity of green tea extract against pathogen adhesion. In: Phytotherapy Research. 2009 ; Vol. 23, No. 4. pp. 460-466.
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