Abstract
Camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract-4 (CSI-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01-0.5 mg/mL. CSI-4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC 50 values (50% inhibition of adhesion) of 0.14-2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI-4 may exert a selective anti-adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria.
| Original language | English |
|---|---|
| Pages (from-to) | 460-466 |
| Number of pages | 7 |
| Journal | Phytotherapy Research |
| Volume | 23 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2009 Apr 1 |
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Keywords
- Anti-adhesive
- Green tea
- Helicobacter pylori
- Propionibacterium acnes
- Staphylococcus aureus
ASJC Scopus subject areas
- Pharmacology
- Medicine(all)
Cite this
In vitro anti-adhesive activity of green tea extract against pathogen adhesion. / Lee, Ji Hye; Shim, Jin Sun; Chung, Mi Sook; Lim, Seung Taik; Kim, Kyung Hyun.
In: Phytotherapy Research, Vol. 23, No. 4, 01.04.2009, p. 460-466.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vitro anti-adhesive activity of green tea extract against pathogen adhesion
AU - Lee, Ji Hye
AU - Shim, Jin Sun
AU - Chung, Mi Sook
AU - Lim, Seung Taik
AU - Kim, Kyung Hyun
PY - 2009/4/1
Y1 - 2009/4/1
N2 - Camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract-4 (CSI-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01-0.5 mg/mL. CSI-4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC 50 values (50% inhibition of adhesion) of 0.14-2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI-4 may exert a selective anti-adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria.
AB - Camellia sinensis polysaccharide has been reported to possess anti-adhesive activity against pathogens. The present study was designed to investigate whether hot water extracts obtained from green tea leaves might inhibit pathogen adhesion to human or mouse cell lines. Green tea extract-4 (CSI-4) with the maximum yield of 4% (w/v) is composed of a major proportion of carbohydrates containing 40% uronic acids, but lack of catechins. It showed strong inhibitory activities against hemagglutination mediated by pathogens Helicobacter pylori, Propionibacterium acnes and Staphylococcus aureus with the minimum inhibitory concentrations of 0.01-0.5 mg/mL. CSI-4 further demonstrated an inhibitory effect on the adhesion of these pathogens to host cell lines with the IC 50 values (50% inhibition of adhesion) of 0.14-2.3 mg/mL. It exhibited the highest activity against P. acnes, but no inhibitory effects were observed against Lactobacillus acidophilus, Bifidobacterium bifidum, Escherichia coli, or Staphylococcus epidermidis. Our results suggest that CSI-4 may exert a selective anti-adhesive effect against certain pathogenic bacteria with no adverse effects against beneficial or commensal bacteria.
KW - Anti-adhesive
KW - Green tea
KW - Helicobacter pylori
KW - Propionibacterium acnes
KW - Staphylococcus aureus
UR - http://www.scopus.com/inward/record.url?scp=64549128728&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64549128728&partnerID=8YFLogxK
U2 - 10.1002/ptr.2609
DO - 10.1002/ptr.2609
M3 - Article
C2 - 19107860
AN - SCOPUS:64549128728
VL - 23
SP - 460
EP - 466
JO - Phytotherapy Research
JF - Phytotherapy Research
SN - 0951-418X
IS - 4
ER -