In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges

Sungyoul Hong, Sung Hwan Kim, Man Hee Rhee, Ae R. Kim, Jee H. Jung, Taehoon Chun, Eun Sook Yoo, Jae Youl Cho

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

(3S,14S)-Petrocortyne A, a lipid compound (a C46 polyacetylenic alcohol), from marine sponges (Petrosia sp.) is potently cytotoxic against several solid tumour cells. In this study, we investigated in vitro anti-inflammatory and pro-aggregative effects of petrocortyne A at non-cytotoxic concentrations on various cellular inflammatory phenomena using the macrophage and monocytic cell lines RAW264.7 and U937. Petrocortyne A blocked tumour necrosis factor-α (TNF-α) production strongly and concentration-dependently in lipopolysaccharide (LPS)-activated RAW264.7 cells and phorbol 12-myristate 13-acetate (PMA)/LPS-treated U937 cells. It also blocked NO production concentration-dependently in LPS-or interferon (IFN)-γ-treated RAW264.7 cells. Among the migration factors tested, the compound selectively blocked the expression of hepatocyte growth factor/scatter factor (HGF/SF). On the other hand, as assessed by a cell-cell adhesion assay, petrocortyne A did not block the activation of adhesion molecules induced by aggregative antibodies to adhesion molecules, but suppressed PMA-induced cell-cell adhesion significantly. Intriguingly, petrocortyne A induced U937 homotypic aggregation following long exposure (2 and 3 days), accompanied by weak induction of pro-aggregative signals such as tyrosine phosphorylation of p132 and phosphorylation of extracellular signal-related kinase 1 and 2 (ERK 1/2). Petrocortyne A may thus inhibit cellular inflammatory processes and immune cell migration to inflamed tissue.

Original languageEnglish
Pages (from-to)448-456
Number of pages9
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume368
Issue number6
DOIs
Publication statusPublished - 2003 Dec 1
Externally publishedYes

Fingerprint

Porifera
Anti-Inflammatory Agents
Lipids
Lipopolysaccharides
Hepatocyte Growth Factor
Cell Adhesion
Petrosia
Acetates
Phosphorylation
U937 Cells
Interferons
Cell Movement
Tyrosine
In Vitro Techniques
petrocortyne A
Phosphotransferases
Tumor Necrosis Factor-alpha
Macrophages
Alcohols
Cell Line

Keywords

  • Anti-inflammatory effects
  • Cell-cell adhesion
  • Lipid compound
  • Marine sponges
  • Petrocortyne A
  • Pro-aggregative effect

ASJC Scopus subject areas

  • Pharmacology

Cite this

In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges. / Hong, Sungyoul; Kim, Sung Hwan; Rhee, Man Hee; Kim, Ae R.; Jung, Jee H.; Chun, Taehoon; Yoo, Eun Sook; Cho, Jae Youl.

In: Naunyn-Schmiedeberg's Archives of Pharmacology, Vol. 368, No. 6, 01.12.2003, p. 448-456.

Research output: Contribution to journalArticle

Hong, Sungyoul ; Kim, Sung Hwan ; Rhee, Man Hee ; Kim, Ae R. ; Jung, Jee H. ; Chun, Taehoon ; Yoo, Eun Sook ; Cho, Jae Youl. / In vitro anti-inflammatory and pro-aggregative effects of a lipid compound, petrocortyne A, from marine sponges. In: Naunyn-Schmiedeberg's Archives of Pharmacology. 2003 ; Vol. 368, No. 6. pp. 448-456.
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AU - Kim, Ae R.

AU - Jung, Jee H.

AU - Chun, Taehoon

AU - Yoo, Eun Sook

AU - Cho, Jae Youl

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