In vitro assessment of the gastrointestinal tolerance and immunomodulatory function of Bacillus methylotrophicus isolated from a traditional Korean fermented soybean food

I. Sim, J. H. Koh, D. J. Kim, S. H. Gu, A. Park, Young Hee Lim

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aims: This study aimed to investigate the potential of Bacillus methylotrophicus as a probiotic. Methods and Results: A Bacillus isolate designated strain C14 was isolated from Korean traditional fermented soybean paste (doenjang). The strain was identified, and its physiological and biochemical properties were characterized. The gastrointestinal tolerance and immunomodulatory function of strain C14 were also investigated. Strain C14 was identified as B. methylotrophicus by analysis of its biochemical properties using the API 50CHB system and by phylogenetic analysis of the 16S rDNA sequence. Strain C14 showed >80% and >75% of survival for artificial gastric juices (pH 2·5 and 1% pepsin) and 0·5% (w/v) bile salt, respectively. Heat-killed B. methylotrophicus C14 inhibited the adhesion of various pathogens and enhanced the adhesion of probiotic bacteria to Caco-2 cells. The heat-killed cells also induced high levels of immune cell proliferation compared with the control and stimulated interleukin-6 and tumour necrosis factor-α production in mouse macrophages. Conclusions: Bacillus methylotrophicus C14 could be used as a probiotic. Significance and Impact of the Study: Recently identified B. methylotrophicus is a new potential probiotic with high gastrointestinal tolerance.

Original languageEnglish
Pages (from-to)718-726
Number of pages9
JournalJournal of Applied Microbiology
Volume118
Issue number3
DOIs
Publication statusPublished - 2015 Mar 1

Fingerprint

Probiotics
Soybeans
Bacillus
Food
Hot Temperature
Gastric Juice
Caco-2 Cells
Pepsin A
Ointments
Ribosomal DNA
Bile Acids and Salts
Interleukin-6
Tumor Necrosis Factor-alpha
Macrophages
Cell Proliferation
Bacteria
In Vitro Techniques

Keywords

  • Bacillus methylotrophicus
  • Acid tolerance
  • Bile tolerance
  • Cytokines
  • Immunomodulatory activity
  • Probiotics

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

In vitro assessment of the gastrointestinal tolerance and immunomodulatory function of Bacillus methylotrophicus isolated from a traditional Korean fermented soybean food. / Sim, I.; Koh, J. H.; Kim, D. J.; Gu, S. H.; Park, A.; Lim, Young Hee.

In: Journal of Applied Microbiology, Vol. 118, No. 3, 01.03.2015, p. 718-726.

Research output: Contribution to journalArticle

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