TY - JOUR
T1 - In vitro effect of clinical propofol concentrations on red blood cell aggregation and deformability
AU - Kim, Yun Hee
AU - Chung, Hye Gyo
AU - Myung, Soohyun A.
AU - Rha, Jee Hyun
AU - Yang, Sung
AU - Nam, Myung Hyun
AU - Shin, Se Hyun
AU - Lim, Choon Hak
PY - 2012
Y1 - 2012
N2 - This in vitro study investigated time-related effects of propofol at the plasma concentrations required for sedation and general anaesthesia, on RBC aggregation, deformability, and morphology. Blood containing propofol at plasma concentrations of 0, 2 and 4 μg ml-1 was incubated in a water bath at 37°C for 1, 2, or 4 hours. RBC elongation indices (EIs) and aggregation indices (AIs), which represent RBC deformability and aggregation, respectively, were measured. Also, RBC morphological indices (MIs), which represent RBC morphology, were calculated. EIs and AIs were similar at propofol concentrations of 0, 2, or 4 μg ml-1 after 1, 2, or 4 hours of incubation. MIs at propofol plasma concentrations 0 or 2 μg ml-1 were similar after 1, 2, and 4 hours of incubation, however, MI at a propofol concentration of 4 μg ml-1 after 4 hours of incubation was higher than its value after 1 or 2 hours of incubation. No significant difference was observed between MIs at propofol plasma concentrations 0, 2, or 4 μg ml-1 after 1, 2, and 4 hours of incubation. At clinical doses, propofol has no direct effects on RBC deformability, aggregation, or morphology over a 4 hours incubation period.
AB - This in vitro study investigated time-related effects of propofol at the plasma concentrations required for sedation and general anaesthesia, on RBC aggregation, deformability, and morphology. Blood containing propofol at plasma concentrations of 0, 2 and 4 μg ml-1 was incubated in a water bath at 37°C for 1, 2, or 4 hours. RBC elongation indices (EIs) and aggregation indices (AIs), which represent RBC deformability and aggregation, respectively, were measured. Also, RBC morphological indices (MIs), which represent RBC morphology, were calculated. EIs and AIs were similar at propofol concentrations of 0, 2, or 4 μg ml-1 after 1, 2, or 4 hours of incubation. MIs at propofol plasma concentrations 0 or 2 μg ml-1 were similar after 1, 2, and 4 hours of incubation, however, MI at a propofol concentration of 4 μg ml-1 after 4 hours of incubation was higher than its value after 1 or 2 hours of incubation. No significant difference was observed between MIs at propofol plasma concentrations 0, 2, or 4 μg ml-1 after 1, 2, and 4 hours of incubation. At clinical doses, propofol has no direct effects on RBC deformability, aggregation, or morphology over a 4 hours incubation period.
KW - Propofol
KW - RBC aggregation
KW - RBC deformability
UR - http://www.scopus.com/inward/record.url?scp=84865727587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84865727587&partnerID=8YFLogxK
U2 - 10.3233/CH-2012-1535
DO - 10.3233/CH-2012-1535
M3 - Article
C2 - 22387482
AN - SCOPUS:84865727587
VL - 51
SP - 287
EP - 292
JO - Clinical Hemorheology and Microcirculation
JF - Clinical Hemorheology and Microcirculation
SN - 1386-0291
IS - 4
ER -