In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant

S. Sarowar, H. Y. Oh, N. I. Hyung, B. W. Min, C. H. Harn, S. K. Yang, S. H. Ok, J. S. Shin

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An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l-1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l-1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Issue number2
Publication statusPublished - 2003 Nov 1



  • Cucurbita species
  • Interspecific hybrid variety
  • Micropropagation
  • Shoot tip multiplication

ASJC Scopus subject areas

  • Horticulture

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