In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant

S. Sarowar, H. Y. Oh, N. I. Hyung, B. W. Min, C. H. Harn, S. K. Yang, S. H. Ok, Jeong Sheop Shin

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

An efficient in vitro micropropagation protocol was developed for direct shoot growth of interspecific Cucurbita hybrid variety using shoot-tips of 5-day-old explants. The excised shoot-tips were cultured on Murashige and Skoog (MS) medium containing two plant growth regulators (6-benzyladenine and naphthaleneacetic acid) with various combinations and concentrations for the study of shoot induction. The best condition for shoot growth was with 3 mg l -1 6-benzyladenine (BA) in MS medium. The shooting frequency was 84% and five shoots were obtained from each explant after 30 days of culture. Shoots (1-1.5 cm length) were rooted most effectively in 1 mg l -1 indole-3 butyric acid (IBA)-supplemented MS medium. The highest root formation rate was 93% and all rooted shoots were transplanted into soil.

Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalPlant Cell, Tissue and Organ Culture
Volume75
Issue number2
DOIs
Publication statusPublished - 2003 Nov 1

Fingerprint

N-benzyladenine
Cucurbita
Plant Roots
micropropagation
Naphthaleneacetic Acids
Plant Growth Regulators
shoots
cultivars
Growth
Soil
benzyladenine
explants
indole butyric acid
plant growth substances
naphthaleneacetic acid
In Vitro Techniques

Keywords

  • Cucurbita species
  • Interspecific hybrid variety
  • Micropropagation
  • Shoot tip multiplication

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant. / Sarowar, S.; Oh, H. Y.; Hyung, N. I.; Min, B. W.; Harn, C. H.; Yang, S. K.; Ok, S. H.; Shin, Jeong Sheop.

In: Plant Cell, Tissue and Organ Culture, Vol. 75, No. 2, 01.11.2003, p. 179-182.

Research output: Contribution to journalArticle

Sarowar, S. ; Oh, H. Y. ; Hyung, N. I. ; Min, B. W. ; Harn, C. H. ; Yang, S. K. ; Ok, S. H. ; Shin, Jeong Sheop. / In vitro micropropagation of a Cucurbita interspecific hybrid cultivar - A root stock plant. In: Plant Cell, Tissue and Organ Culture. 2003 ; Vol. 75, No. 2. pp. 179-182.
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