In vitro model using mouse hepatocytes for study of alcohol stress

Jae Won Yang, Jun Seop Shin, Jae Jeong Lee, Hyo Ihl Chang, Chan Wha Kim

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

In this study, the effects of ethanol and allyl alcohol on primary mouse hepatocytes were investigated. No cytotoxicity was observed by ethanol treatments, but more toxicity to cells was found in the response to allyl alcohol treatment. The expression of cytochrome P450 2E1 (CYP2E1), phase I enzyme was examined in response to ethanol and allyl alcohol. Both xenobiotics induced CYP2E1 up to 1.5 ∼ 5 fold at the protein level. The effects of insulin on CYP2E1 expression were also measured. Insulin, which has been regarded as an essential hormone for primary hepatocytes, was shown to decrease the level of CYP2E1 protein, and did not affect cell viability. These results on CYP2E1 induction demonstrate that primary mouse hepatocytes, when using ethanol and allyl alcohol as substrates and in insulin-free medium, provide a suitable system for the studies of the role of CYP2E1 in xenobiotic metabolism and toxicity.

Original languageEnglish
Pages (from-to)1528-1533
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number7
DOIs
Publication statusPublished - 2001 Jul

Keywords

  • Allyl alcohol
  • CYP2E1
  • Ethanol
  • Hepatotoxicity
  • Mouse hepatocyte

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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