In vitro monitoring of cardiomyogenic differentiation of mesenchymal stem cells using sodium iodide symporter gene

Min Hwan Kim, Yong Jin Lee, Kwang Il Kim, Tae Sup Lee, Kwang Sun Woo, Dong Soo Lee, Chan Wha Kim, Chang Woon Choi, Sang Moo Lim, Joo Hyun Kang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

There is a need for non-invasive monitoring the differentiation of transplanted cell in stem cell therapy. In this study, we evaluated the usefulness of sodium iodide symporter (NIS) gene as molecular imaging reporter gene during cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells (BMSCs). In a previous study, we showed that a transgenic mouse model expressing NIS driven by the α-myosin heavy chain (α-MHC) promoter was developed to image cardiomyocytes in vivo with a γ-camera and micro positron emission tomography (microPET). BMSCs were found to express stem cell specific surface markers including stem cell antigen-1 (Sca- 1) and CD44. After treatment with all-trans retinoic acid (ATRA), BMSCs morphologically changed and acquired a cardiomyocyte-like shape. The cardiomyogenic differentiation of BMSCs resulted in upregulated expression of cardiac specific genes including α-MHC and ventricular myosin light chain 2 (MLC-2v) and decreased expression of the stem cell specific marker, Sca-1. The ATRA treated BMSCs exhibited higher 125I uptake than that of nontreated cells, and this result suggested that the cardiomyogenic differentiation upregulated NIS gene activity as reporter in vitro. We demonstrated that, under the control of the a-MHC promoter, NIS activity reflected cardiomyogenic differentiation of BMSCs in vitro, and an imaging system based on NIS, the reporter gene, may be a useful tool for monitoring in the lineage specific differentiation of stem cells.

Original languageEnglish
Pages (from-to)304-310
Number of pages7
JournalTissue Engineering and Regenerative Medicine
Volume9
Issue number6
DOIs
Publication statusPublished - 2012 Dec

Keywords

  • Alpha-myosin heavy chain
  • Bone marrow derived mesenchymal stem cells
  • Cardiomyogenic differentiation
  • Sodium iodide symporter
  • Transgenic mouse

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomedical Engineering

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  • Cite this

    Kim, M. H., Lee, Y. J., Kim, K. I., Lee, T. S., Woo, K. S., Lee, D. S., Kim, C. W., Choi, C. W., Lim, S. M., & Kang, J. H. (2012). In vitro monitoring of cardiomyogenic differentiation of mesenchymal stem cells using sodium iodide symporter gene. Tissue Engineering and Regenerative Medicine, 9(6), 304-310. https://doi.org/10.1007/s13770-012-0003-y