TY - JOUR
T1 - In vitro monitoring of cardiomyogenic differentiation of mesenchymal stem cells using sodium iodide symporter gene
AU - Kim, Min Hwan
AU - Lee, Yong Jin
AU - Kim, Kwang Il
AU - Lee, Tae Sup
AU - Woo, Kwang Sun
AU - Lee, Dong Soo
AU - Kim, Chan Wha
AU - Choi, Chang Woon
AU - Lim, Sang Moo
AU - Kang, Joo Hyun
PY - 2012/12
Y1 - 2012/12
N2 - There is a need for non-invasive monitoring the differentiation of transplanted cell in stem cell therapy. In this study, we evaluated the usefulness of sodium iodide symporter (NIS) gene as molecular imaging reporter gene during cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells (BMSCs). In a previous study, we showed that a transgenic mouse model expressing NIS driven by the α-myosin heavy chain (α-MHC) promoter was developed to image cardiomyocytes in vivo with a γ-camera and micro positron emission tomography (microPET). BMSCs were found to express stem cell specific surface markers including stem cell antigen-1 (Sca- 1) and CD44. After treatment with all-trans retinoic acid (ATRA), BMSCs morphologically changed and acquired a cardiomyocyte-like shape. The cardiomyogenic differentiation of BMSCs resulted in upregulated expression of cardiac specific genes including α-MHC and ventricular myosin light chain 2 (MLC-2v) and decreased expression of the stem cell specific marker, Sca-1. The ATRA treated BMSCs exhibited higher 125I uptake than that of nontreated cells, and this result suggested that the cardiomyogenic differentiation upregulated NIS gene activity as reporter in vitro. We demonstrated that, under the control of the a-MHC promoter, NIS activity reflected cardiomyogenic differentiation of BMSCs in vitro, and an imaging system based on NIS, the reporter gene, may be a useful tool for monitoring in the lineage specific differentiation of stem cells.
AB - There is a need for non-invasive monitoring the differentiation of transplanted cell in stem cell therapy. In this study, we evaluated the usefulness of sodium iodide symporter (NIS) gene as molecular imaging reporter gene during cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells (BMSCs). In a previous study, we showed that a transgenic mouse model expressing NIS driven by the α-myosin heavy chain (α-MHC) promoter was developed to image cardiomyocytes in vivo with a γ-camera and micro positron emission tomography (microPET). BMSCs were found to express stem cell specific surface markers including stem cell antigen-1 (Sca- 1) and CD44. After treatment with all-trans retinoic acid (ATRA), BMSCs morphologically changed and acquired a cardiomyocyte-like shape. The cardiomyogenic differentiation of BMSCs resulted in upregulated expression of cardiac specific genes including α-MHC and ventricular myosin light chain 2 (MLC-2v) and decreased expression of the stem cell specific marker, Sca-1. The ATRA treated BMSCs exhibited higher 125I uptake than that of nontreated cells, and this result suggested that the cardiomyogenic differentiation upregulated NIS gene activity as reporter in vitro. We demonstrated that, under the control of the a-MHC promoter, NIS activity reflected cardiomyogenic differentiation of BMSCs in vitro, and an imaging system based on NIS, the reporter gene, may be a useful tool for monitoring in the lineage specific differentiation of stem cells.
KW - Alpha-myosin heavy chain
KW - Bone marrow derived mesenchymal stem cells
KW - Cardiomyogenic differentiation
KW - Sodium iodide symporter
KW - Transgenic mouse
UR - http://www.scopus.com/inward/record.url?scp=84881393625&partnerID=8YFLogxK
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U2 - 10.1007/s13770-012-0003-y
DO - 10.1007/s13770-012-0003-y
M3 - Article
AN - SCOPUS:84881393625
VL - 9
SP - 304
EP - 310
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
SN - 1738-2696
IS - 6
ER -