In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines

Jinlong Yin, Xun Jin, Samuel Beck, Dong Ho Kang, Zhongshan Hong, Zhehu Li, Yongcheng Jin, Qiankun Zhang, Yun Jaie Choi, Sung Chan Kim, Hyunggee Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalBiotechnology Letters
Volume32
Issue number2
DOIs
Publication statusPublished - 2010 Jan 1

Keywords

  • Adipogenesis
  • BEFS-MyoD
  • BEFS-PPARγ2
  • Bovine embryonic fibroblast cell line
  • Myogenesis

ASJC Scopus subject areas

  • Biotechnology

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  • Cite this

    Yin, J., Jin, X., Beck, S., Kang, D. H., Hong, Z., Li, Z., Jin, Y., Zhang, Q., Choi, Y. J., Kim, S. C., & Kim, H. (2010). In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines. Biotechnology Letters, 32(2), 195-202. https://doi.org/10.1007/s10529-009-0142-y