In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines

Jinlong Yin, Xun Jin, Samuel Beck, Dong Ho Kang, Zhongshan Hong, Zhehu Li, Yongcheng Jin, Qiankun Zhang, Yun Jaie Choi, Sung Chan Kim, Hyunggee Kim

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.

Original languageEnglish
Pages (from-to)195-202
Number of pages8
JournalBiotechnology Letters
Volume32
Issue number2
DOIs
Publication statusPublished - 2010 Jan 1

Fingerprint

Fibroblasts
Cell Line
troglitazone
In Vitro Techniques
Muscles
Biological Models
Adipocytes
Culture Media
Adipose Tissue
Ligands

Keywords

  • Adipogenesis
  • BEFS-MyoD
  • BEFS-PPARγ2
  • Bovine embryonic fibroblast cell line
  • Myogenesis

ASJC Scopus subject areas

  • Biotechnology

Cite this

In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines. / Yin, Jinlong; Jin, Xun; Beck, Samuel; Kang, Dong Ho; Hong, Zhongshan; Li, Zhehu; Jin, Yongcheng; Zhang, Qiankun; Choi, Yun Jaie; Kim, Sung Chan; Kim, Hyunggee.

In: Biotechnology Letters, Vol. 32, No. 2, 01.01.2010, p. 195-202.

Research output: Contribution to journalArticle

Yin, J, Jin, X, Beck, S, Kang, DH, Hong, Z, Li, Z, Jin, Y, Zhang, Q, Choi, YJ, Kim, SC & Kim, H 2010, 'In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines', Biotechnology Letters, vol. 32, no. 2, pp. 195-202. https://doi.org/10.1007/s10529-009-0142-y
Yin, Jinlong ; Jin, Xun ; Beck, Samuel ; Kang, Dong Ho ; Hong, Zhongshan ; Li, Zhehu ; Jin, Yongcheng ; Zhang, Qiankun ; Choi, Yun Jaie ; Kim, Sung Chan ; Kim, Hyunggee. / In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines. In: Biotechnology Letters. 2010 ; Vol. 32, No. 2. pp. 195-202.
@article{a8e379b3d25c477f916af22cdadbb208,
title = "In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines",
abstract = "Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.",
keywords = "Adipogenesis, BEFS-MyoD, BEFS-PPARγ2, Bovine embryonic fibroblast cell line, Myogenesis",
author = "Jinlong Yin and Xun Jin and Samuel Beck and Kang, {Dong Ho} and Zhongshan Hong and Zhehu Li and Yongcheng Jin and Qiankun Zhang and Choi, {Yun Jaie} and Kim, {Sung Chan} and Hyunggee Kim",
year = "2010",
month = "1",
day = "1",
doi = "10.1007/s10529-009-0142-y",
language = "English",
volume = "32",
pages = "195--202",
journal = "Biotechnology Letters",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "2",

}

TY - JOUR

T1 - In vitro myogenic and adipogenic differentiation model of genetically engineered bovine embryonic fibroblast cell lines

AU - Yin, Jinlong

AU - Jin, Xun

AU - Beck, Samuel

AU - Kang, Dong Ho

AU - Hong, Zhongshan

AU - Li, Zhehu

AU - Jin, Yongcheng

AU - Zhang, Qiankun

AU - Choi, Yun Jaie

AU - Kim, Sung Chan

AU - Kim, Hyunggee

PY - 2010/1/1

Y1 - 2010/1/1

N2 - Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.

AB - Our current understanding of muscle and adipose tissue development has been largely restricted to the study of murine myogenic and adipogenic cell lines, since attempts to establish these cell lines from other species have met with only limited success. Here we report that a spontaneously immortalized bovine embryonic fibroblast cell line (BEFS) undergoes differentiation into adipogenic or myogenic lineages when ectopically transduced with PPARγ2 (an adipogenic lineage determinant) or MyoD (a myogenic lineage determinant) and grown in adipogenic and myogenic differentiation culture media (ADCM and MDCM, respectively). We also found that PPARγ2-overexpressing BEFS cells (BEFS-PPARγ2) grown in ADCM with or without the PPARγ2 ligand, troglitazone, preferentially differentiate into adipogenic cells in the presence of ectopic MyoD expression. Ectopic expression of PPARγ2 in the inducible MyoD-overepxressing BEFS cells (BEFS-TetOn-MyoD) completely suppresses myogenic differentiation and leads to a significant increase in adipogenic differentiation, suggesting that the adipogenic differentiation program might be dominant. Therefore, BEFS, BEFS-PPARγ2, and BEFS-TetOn-MyoD would be a valuable biological model for understanding a fundamental principle underlying myogenic and adipogenic development, and for isolating various genetic and chemical factors that enable muscle and adipocyte differentiation.

KW - Adipogenesis

KW - BEFS-MyoD

KW - BEFS-PPARγ2

KW - Bovine embryonic fibroblast cell line

KW - Myogenesis

UR - http://www.scopus.com/inward/record.url?scp=76449088089&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76449088089&partnerID=8YFLogxK

U2 - 10.1007/s10529-009-0142-y

DO - 10.1007/s10529-009-0142-y

M3 - Article

VL - 32

SP - 195

EP - 202

JO - Biotechnology Letters

JF - Biotechnology Letters

SN - 0141-5492

IS - 2

ER -