In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages

Bong Gu Yi, Ok Kyu Park, Myeong Seon Jeong, Seung Hae Kwon, Jae In Jung, Seongsoo Lee, Sungwoo Ryoo, Sung Eun Kim, Jin Won Kim, Won Jin Moon, Kyeongsoon Park

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Scavenger receptors (SRs) expressed on the activated macrophages in inflammation sites have been considered as the most interesting and important target biomarker for targeted drug delivery, imaging and therapy. In the present study, we fabricated the scavenger receptor-A (SR-A) targeted-photoactivatable nanoagents (termed as Ce6/DS-DOCA) by entrapping chlorin e6 (Ce6) into the amphiphilic dextran sulfate-deoxycholic acid (DS-DOCA) conjugates via physically hydrophobic interactions. Insoluble Ce6 was easily encapsulated into DS-DOCA nanoparticles by a dialysis method and the loading efficiency was approximately 51.7%. The Ce6/DS-DOCA formed nano-sized self-assembled aggregates (28.8 ± 5.6 nm in diameter), confirmed by transmission electron microscope, UV/Vis and fluorescence spectrophotometer. The Ce6/DS-DOCA nanoagents could generate highly reactive singlet oxygen under laser irradiation. Also, in vitro studies showed that they were more specifically taken up by lipopolysaccharide (LPS)-induced activated macrophages (RAW 264.7) via a SR-A-mediated endocytosis, relative to by non-activated macrophages, and notably induced cell death of activated macrophages under laser irradiation. Therefore, SR-A targetable and photoactivatable Ce6/DS-DOCA nanoagents with more selective targeting to the activated macrophages will have great potential for treatment of inflammatory diseases.

Original languageEnglish
Pages (from-to)181-189
Number of pages9
JournalInternational Journal of Biological Macromolecules
Volume97
DOIs
Publication statusPublished - 2017 Apr 1

Keywords

  • Activated macrophages
  • Photoactivatable nanoagents
  • Scavenger receptor targeting

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology

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  • Cite this

    Yi, B. G., Park, O. K., Jeong, M. S., Kwon, S. H., Jung, J. I., Lee, S., Ryoo, S., Kim, S. E., Kim, J. W., Moon, W. J., & Park, K. (2017). In vitro photodynamic effects of scavenger receptor targeted-photoactivatable nanoagents on activated macrophages. International Journal of Biological Macromolecules, 97, 181-189. https://doi.org/10.1016/j.ijbiomac.2017.01.037