In vitro trans-differentiation of rat mesenchymal cells into insulin-producing cells by rat pancreatic extract

Kyung Suk Choi, Jun Seop Shin, Jae Jeong Lee, Young Soo Kim, Seung Bum Kim, Chan Wha Kim

Research output: Contribution to journalArticle

153 Citations (Scopus)

Abstract

Recent reports have suggested that mesenchymal cells derived from bone marrow may differentiate into not only mesenchymal lineage cells but also other lineage cells. There is possibility for insulin-producing cells (IPCs) to be differentiated from mesenchymal cells. We used self-functional repair stimuli of stem cells by partial injury. Rat pancreatic extract (RPE) from the regenerating pancreas (2 days after 60% pancreatectomy) was treated to rat mesenchymal cells. After the treatment of RPE, they made clusters like islet of Langerhans within a week and expressed four pancreatic endocrine hormones; insulin, glucagon, pancreatic polypeptide, and somatostatin. Moreover, IPCs released insulin in response to normal glucose challenge. Here we demonstrate that the treatment of RPE can differentiate rat mesenchymal cells into IPCs which can be a potential source for the therapy of diabetes.

Original languageEnglish
Pages (from-to)1299-1305
Number of pages7
JournalBiochemical and biophysical research communications
Volume330
Issue number4
DOIs
Publication statusPublished - 2005 May 20

Keywords

  • Bone marrow stromal cell
  • Differentiation
  • Mesenchymal stem cell
  • Pancreatectomy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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