In vivo antifungal effects of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi

Chi Hoon Lee, Hojoung Lee, Ju Hyun Jeon, Hoi Seon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent LC50 value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an LC50 value of 210, 20, 180, and 290 mg/l against B. cinerea, E graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an LC50 value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

Original languageEnglish
Pages (from-to)1402-1407
Number of pages6
JournalJournal of Microbiology and Biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec

Fingerprint

Pyricularia grisea
Coptis
Fungicides
Alkaloids
Fungi
Butanols
Chloroform
Chlorine compounds
Butenes
Berberine Alkaloids
Emodin
Berberine
Curcumin
Pathogens
Chlorides
Spectrum analysis
Crops
Phytophthora infestans
Rhizoctonia
Botrytis

Keywords

  • Berberine chloride
  • Coptis japonica
  • Coptisine chloride
  • Fungicidal activity
  • Palmatine iodide

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

In vivo antifungal effects of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi. / Lee, Chi Hoon; Lee, Hojoung; Jeon, Ju Hyun; Lee, Hoi Seon.

In: Journal of Microbiology and Biotechnology, Vol. 15, No. 6, 12.2005, p. 1402-1407.

Research output: Contribution to journalArticle

@article{1baf8b1dc8074993817b38bd16bca4ba,
title = "In vivo antifungal effects of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi",
abstract = "The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent LC50 value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an LC50 value of 210, 20, 180, and 290 mg/l against B. cinerea, E graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an LC50 value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.",
keywords = "Berberine chloride, Coptis japonica, Coptisine chloride, Fungicidal activity, Palmatine iodide",
author = "Lee, {Chi Hoon} and Hojoung Lee and Jeon, {Ju Hyun} and Lee, {Hoi Seon}",
year = "2005",
month = "12",
language = "English",
volume = "15",
pages = "1402--1407",
journal = "Journal of Microbiology and Biotechnology",
issn = "1017-7825",
publisher = "Korean Society for Microbiolog and Biotechnology",
number = "6",

}

TY - JOUR

T1 - In vivo antifungal effects of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi

AU - Lee, Chi Hoon

AU - Lee, Hojoung

AU - Jeon, Ju Hyun

AU - Lee, Hoi Seon

PY - 2005/12

Y1 - 2005/12

N2 - The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent LC50 value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an LC50 value of 210, 20, 180, and 290 mg/l against B. cinerea, E graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an LC50 value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

AB - The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent LC50 value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an LC50 value of 210, 20, 180, and 290 mg/l against B. cinerea, E graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an LC50 value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

KW - Berberine chloride

KW - Coptis japonica

KW - Coptisine chloride

KW - Fungicidal activity

KW - Palmatine iodide

UR - http://www.scopus.com/inward/record.url?scp=33646739939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33646739939&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:33646739939

VL - 15

SP - 1402

EP - 1407

JO - Journal of Microbiology and Biotechnology

JF - Journal of Microbiology and Biotechnology

SN - 1017-7825

IS - 6

ER -