In vivo antifungal effects of Coptis japonica root-derived isoquinoline alkaloids against phytopathogenic fungi

Chi Hoon Lee, Hoi Joung Lee, Ju Hyun Jeon, Hoi Seon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent LC50 value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an LC50 value of 210, 20, 180, and 290 mg/l against B. cinerea, E graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an LC50 value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

Original languageEnglish
Pages (from-to)1402-1407
Number of pages6
JournalJournal of microbiology and biotechnology
Volume15
Issue number6
Publication statusPublished - 2005 Dec 1

Keywords

  • Berberine chloride
  • Coptis japonica
  • Coptisine chloride
  • Fungicidal activity
  • Palmatine iodide

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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