In vivo assay of the potential gadolinium-induced toxicity for sensory hair cells using a zebrafish animal model

Yoon Chan Rah, Eun Jung Han, Saemi Park, Jihye Rhee, Soonil Koun, Hae Chul Park, June Choi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Recently, intratympanic injection of gadolinium-based contrast agent (GdC) is growing in use to visualize the endolymphatic hydrops. Although GdC has been quite safely used over 20 years through intravenous injection, the biological influence of GdC on sensory hair cells needs to be thoroughly assessed for wider clinical application of it through intratympanic injection. In this in vivo experimental study, the summated number of sensory hair cells (SO1, SO2, O1 and OC1 neuromasts) showed a steep decrease in the group exposed to 10% and 20% GdC (35.7 ± 7.3, 15.09 ± 10.82, respectively, P <.01) compared with the control group (47.18 ± 2.30). An increase in apoptosis was also observed in the group exposed to 20% gadolinium (7.20 ± 5.56), as compared with the control group (0.08 ± 0.72) or the group exposed to 10% gadolinium (3.48 ± 3.32). A significant reduction in the viable cytoplasmic mitochondria was observed in embryos exposed to 20% GdC (369 ± 124 μm2, P =.01) as compared with control embryos (447 ± 118 μm2) or embryos exposed to 10% GdC (420 ± 108 μm2). GdC administration did not impact peripheral neural structures. GdC caused a significant reduction in sensory hair cell counts in response to high concentrations along with increased apoptosis and mitochondrial damage. However, it may not be likely that GdC will lead to hair cell toxicity, as the estimated concentration in the inner ear after clinically tried intratympanic injection is far more diluted than the non-toxic concentration (0.625%) that was tested in this study.

Original languageEnglish
Pages (from-to)1398-1404
Number of pages7
JournalJournal of Applied Toxicology
Volume38
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1

Fingerprint

Gadolinium
Zebrafish
Toxicity
Assays
Animals
Animal Models
Contrast Media
Cells
Embryonic Structures
Endolymphatic Hydrops
Apoptosis
Control Groups
Mitochondria
Inner Ear
Intravenous Injections
Cell Count

Keywords

  • gadolinium
  • hair cell
  • inner ear
  • ototoxicity
  • zebrafish

ASJC Scopus subject areas

  • Toxicology

Cite this

In vivo assay of the potential gadolinium-induced toxicity for sensory hair cells using a zebrafish animal model. / Rah, Yoon Chan; Han, Eun Jung; Park, Saemi; Rhee, Jihye; Koun, Soonil; Park, Hae Chul; Choi, June.

In: Journal of Applied Toxicology, Vol. 38, No. 11, 01.11.2018, p. 1398-1404.

Research output: Contribution to journalArticle

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abstract = "Recently, intratympanic injection of gadolinium-based contrast agent (GdC) is growing in use to visualize the endolymphatic hydrops. Although GdC has been quite safely used over 20 years through intravenous injection, the biological influence of GdC on sensory hair cells needs to be thoroughly assessed for wider clinical application of it through intratympanic injection. In this in vivo experimental study, the summated number of sensory hair cells (SO1, SO2, O1 and OC1 neuromasts) showed a steep decrease in the group exposed to 10{\%} and 20{\%} GdC (35.7 ± 7.3, 15.09 ± 10.82, respectively, P <.01) compared with the control group (47.18 ± 2.30). An increase in apoptosis was also observed in the group exposed to 20{\%} gadolinium (7.20 ± 5.56), as compared with the control group (0.08 ± 0.72) or the group exposed to 10{\%} gadolinium (3.48 ± 3.32). A significant reduction in the viable cytoplasmic mitochondria was observed in embryos exposed to 20{\%} GdC (369 ± 124 μm2, P =.01) as compared with control embryos (447 ± 118 μm2) or embryos exposed to 10{\%} GdC (420 ± 108 μm2). GdC administration did not impact peripheral neural structures. GdC caused a significant reduction in sensory hair cell counts in response to high concentrations along with increased apoptosis and mitochondrial damage. However, it may not be likely that GdC will lead to hair cell toxicity, as the estimated concentration in the inner ear after clinically tried intratympanic injection is far more diluted than the non-toxic concentration (0.625{\%}) that was tested in this study.",
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AU - Park, Hae Chul

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