In vivo assessment of hair cell damage and developmental toxicity caused by gestational caffeine exposure using zebrafish (Danio rerio) models

Yoon Chan Rah, Myung Hoon Yoo, June Choi, Saemi Park, Hae Chul Park, Kyoung Ho Oh, Seung Hoon Lee, Soon Young Kwon

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6 Citations (Scopus)

Abstract

The aim of the present study was to evaluate hair cell damage and associated developmental toxicity caused by gestational caffeine exposure. We exposed embryos to various caffeine concentrations (25 μM, 125 μM, 250 μM, and 500 μM) and evaluated developmental toxicity of the embryos at 72 and 120 h and hair cell damage at 120 h after fertilization. The average number of total hair cells within four neuromasts exposed to various concentrations of caffeine was compared with that of the control group. To seek the underlying mechanisms, TUNEL and DASPEI assay were carried out to evaluate hair cell apoptosis and mitochondrial damage, respectively. Morphologic abnormality, mortality, hatching rate, and heart rate were also evaluated. Caffeine induced significant hair cell damage compared with control group (p < 0.01, control; 35.64 ± 10.48 cells, 500 μM caffeine; 23.32 ± 12.14 cells, n = 25–30). Significant increase in the hair cell apoptosis was confirmed in a dose-dependent manner (p < 0.01, TUNEL assay) and the mitochondrial damage in high caffeine concentrations (250, 500 μM) (p < 0.01, DASPEI assay).Morphologic abnormalities were significantly increased in high caffeine concentrations (250 or 500 μM) for body shape, notochord, and heart at both 3-, and 5-dpf. The control group exhibited 3.3% mortality which increased up to 11.6% at 500 μM caffeine. Rapid hatching was present at 48 h (control; 46.6%, 500 μM caffeine; 100%). In conclusion, gestational caffeine exposure caused significant hair cell damage and developmental toxicities in zebrafish at early developmental stages.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalNeurotoxicology and Teratology
Volume64
DOIs
Publication statusPublished - 2017 Nov 1

Fingerprint

Zebrafish
Caffeine
Toxicity
Cells
Assays
In Situ Nick-End Labeling
Control Groups
Embryonic Structures
Apoptosis
Notochord
Mortality
Fertilization
Heart Rate

Keywords

  • Caffeine
  • Embryotoxicity
  • Hair cell
  • Teratogenicity
  • Zebrafish

ASJC Scopus subject areas

  • Toxicology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

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title = "In vivo assessment of hair cell damage and developmental toxicity caused by gestational caffeine exposure using zebrafish (Danio rerio) models",
abstract = "The aim of the present study was to evaluate hair cell damage and associated developmental toxicity caused by gestational caffeine exposure. We exposed embryos to various caffeine concentrations (25 μM, 125 μM, 250 μM, and 500 μM) and evaluated developmental toxicity of the embryos at 72 and 120 h and hair cell damage at 120 h after fertilization. The average number of total hair cells within four neuromasts exposed to various concentrations of caffeine was compared with that of the control group. To seek the underlying mechanisms, TUNEL and DASPEI assay were carried out to evaluate hair cell apoptosis and mitochondrial damage, respectively. Morphologic abnormality, mortality, hatching rate, and heart rate were also evaluated. Caffeine induced significant hair cell damage compared with control group (p < 0.01, control; 35.64 ± 10.48 cells, 500 μM caffeine; 23.32 ± 12.14 cells, n = 25–30). Significant increase in the hair cell apoptosis was confirmed in a dose-dependent manner (p < 0.01, TUNEL assay) and the mitochondrial damage in high caffeine concentrations (250, 500 μM) (p < 0.01, DASPEI assay).Morphologic abnormalities were significantly increased in high caffeine concentrations (250 or 500 μM) for body shape, notochord, and heart at both 3-, and 5-dpf. The control group exhibited 3.3{\%} mortality which increased up to 11.6{\%} at 500 μM caffeine. Rapid hatching was present at 48 h (control; 46.6{\%}, 500 μM caffeine; 100{\%}). In conclusion, gestational caffeine exposure caused significant hair cell damage and developmental toxicities in zebrafish at early developmental stages.",
keywords = "Caffeine, Embryotoxicity, Hair cell, Teratogenicity, Zebrafish",
author = "Rah, {Yoon Chan} and Yoo, {Myung Hoon} and June Choi and Saemi Park and Park, {Hae Chul} and Oh, {Kyoung Ho} and Lee, {Seung Hoon} and Kwon, {Soon Young}",
year = "2017",
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language = "English",
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TY - JOUR

T1 - In vivo assessment of hair cell damage and developmental toxicity caused by gestational caffeine exposure using zebrafish (Danio rerio) models

AU - Rah, Yoon Chan

AU - Yoo, Myung Hoon

AU - Choi, June

AU - Park, Saemi

AU - Park, Hae Chul

AU - Oh, Kyoung Ho

AU - Lee, Seung Hoon

AU - Kwon, Soon Young

PY - 2017/11/1

Y1 - 2017/11/1

N2 - The aim of the present study was to evaluate hair cell damage and associated developmental toxicity caused by gestational caffeine exposure. We exposed embryos to various caffeine concentrations (25 μM, 125 μM, 250 μM, and 500 μM) and evaluated developmental toxicity of the embryos at 72 and 120 h and hair cell damage at 120 h after fertilization. The average number of total hair cells within four neuromasts exposed to various concentrations of caffeine was compared with that of the control group. To seek the underlying mechanisms, TUNEL and DASPEI assay were carried out to evaluate hair cell apoptosis and mitochondrial damage, respectively. Morphologic abnormality, mortality, hatching rate, and heart rate were also evaluated. Caffeine induced significant hair cell damage compared with control group (p < 0.01, control; 35.64 ± 10.48 cells, 500 μM caffeine; 23.32 ± 12.14 cells, n = 25–30). Significant increase in the hair cell apoptosis was confirmed in a dose-dependent manner (p < 0.01, TUNEL assay) and the mitochondrial damage in high caffeine concentrations (250, 500 μM) (p < 0.01, DASPEI assay).Morphologic abnormalities were significantly increased in high caffeine concentrations (250 or 500 μM) for body shape, notochord, and heart at both 3-, and 5-dpf. The control group exhibited 3.3% mortality which increased up to 11.6% at 500 μM caffeine. Rapid hatching was present at 48 h (control; 46.6%, 500 μM caffeine; 100%). In conclusion, gestational caffeine exposure caused significant hair cell damage and developmental toxicities in zebrafish at early developmental stages.

AB - The aim of the present study was to evaluate hair cell damage and associated developmental toxicity caused by gestational caffeine exposure. We exposed embryos to various caffeine concentrations (25 μM, 125 μM, 250 μM, and 500 μM) and evaluated developmental toxicity of the embryos at 72 and 120 h and hair cell damage at 120 h after fertilization. The average number of total hair cells within four neuromasts exposed to various concentrations of caffeine was compared with that of the control group. To seek the underlying mechanisms, TUNEL and DASPEI assay were carried out to evaluate hair cell apoptosis and mitochondrial damage, respectively. Morphologic abnormality, mortality, hatching rate, and heart rate were also evaluated. Caffeine induced significant hair cell damage compared with control group (p < 0.01, control; 35.64 ± 10.48 cells, 500 μM caffeine; 23.32 ± 12.14 cells, n = 25–30). Significant increase in the hair cell apoptosis was confirmed in a dose-dependent manner (p < 0.01, TUNEL assay) and the mitochondrial damage in high caffeine concentrations (250, 500 μM) (p < 0.01, DASPEI assay).Morphologic abnormalities were significantly increased in high caffeine concentrations (250 or 500 μM) for body shape, notochord, and heart at both 3-, and 5-dpf. The control group exhibited 3.3% mortality which increased up to 11.6% at 500 μM caffeine. Rapid hatching was present at 48 h (control; 46.6%, 500 μM caffeine; 100%). In conclusion, gestational caffeine exposure caused significant hair cell damage and developmental toxicities in zebrafish at early developmental stages.

KW - Caffeine

KW - Embryotoxicity

KW - Hair cell

KW - Teratogenicity

KW - Zebrafish

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JO - Neurotoxicology and Teratology

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