Abstract
We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.
| Original language | English |
|---|---|
| Pages (from-to) | 55-62 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 344 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2006 May 26 |
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Keywords
- CabZIP1
- CaPR-1 promoter
- Chromatin immunoprecipitation (ChIP)
- Hot pepper (Capsicum annuum L.)
- Nuclear localization
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter. / Lee, Boo Ja; Park, Chang Jin; Kim, Sung Kyu; Kim, Ki Jeong; Paek, Kyung Hee.
In: Biochemical and Biophysical Research Communications, Vol. 344, No. 1, 26.05.2006, p. 55-62.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter
AU - Lee, Boo Ja
AU - Park, Chang Jin
AU - Kim, Sung Kyu
AU - Kim, Ki Jeong
AU - Paek, Kyung Hee
PY - 2006/5/26
Y1 - 2006/5/26
N2 - We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.
AB - We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.
KW - CabZIP1
KW - CaPR-1 promoter
KW - Chromatin immunoprecipitation (ChIP)
KW - Hot pepper (Capsicum annuum L.)
KW - Nuclear localization
UR - http://www.scopus.com/inward/record.url?scp=33646486277&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646486277&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2006.03.153
DO - 10.1016/j.bbrc.2006.03.153
M3 - Article
VL - 344
SP - 55
EP - 62
JO - The BMJ
JF - The BMJ
SN - 0730-6512
IS - 1
ER -