In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter

Boo Ja Lee, Chang Jin Park, Sung Kyu Kim, Ki Jeong Kim, Kyung Hee Paek

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We find that salicylic acid and ethephon treatment in hot pepper increases the expression of a putative basic/leucine zipper (bZIP) transcription factor gene, CabZIP1. CabZIP1 mRNA is expressed ubiquitously in various organs. The green fluorescent protein-fused transcription factor, CabZIP1::GFP, can be specifically localized to the nucleus, an action that is consistent with the presence of a nuclear localization signal in its protein sequence. Transient overexpression of the CabZIP1 transcription factor results in an increase in PR-1 transcripts level in Nicotiana benthamiana leaves. Using chromatin immunoprecipitation, we demonstrate that CabZIP1 binds to the G-box elements in native promoter of the hot pepper pathogenesis-related protein 1 (CaPR-1) gene in vivo. Taken together, our results suggest that CabZIP1 plays a role as a transcriptional regulator of the CaPR-1 gene.

Original languageEnglish
Pages (from-to)55-62
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number1
DOIs
Publication statusPublished - 2006 May 26

Fingerprint

Basic-Leucine Zipper Transcription Factors
Capsicum
Genes
Transcription Factors
Nuclear Localization Signals
Proteins
Salicylic Acid
Chromatin Immunoprecipitation
Green Fluorescent Proteins
Chromatin
Tobacco
Messenger RNA

Keywords

  • CabZIP1
  • CaPR-1 promoter
  • Chromatin immunoprecipitation (ChIP)
  • Hot pepper (Capsicum annuum L.)
  • Nuclear localization

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

In vivo binding of hot pepper bZIP transcription factor CabZIP1 to the G-box region of pathogenesis-related protein 1 promoter. / Lee, Boo Ja; Park, Chang Jin; Kim, Sung Kyu; Kim, Ki Jeong; Paek, Kyung Hee.

In: Biochemical and Biophysical Research Communications, Vol. 344, No. 1, 26.05.2006, p. 55-62.

Research output: Contribution to journalArticle

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AU - Paek, Kyung Hee

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