Abstract
Tubular scaffolds were fabricated from very elastic poly(L-lactide-co- ε-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, masson trichrome staining, SM α-actin antibody, and CM-DiI labeling confirmed that the regular morphology and biofunction of the SMCs seeded and the expression of the vascular smooth muscle matrices in PLCL scaffolds. The implanted PLCL scaffolds displayed a slow degradation on time, where caprolactone units were faster degraded than lactide did. This could be explained by the fact that amorphous regions composed of mainly CL moieties degraded earlier than hard domains where most of the LA units were located. From these results, the scaffolds applied in this study were found to exhibit excellent tissue compatibility to SMCs and might be very useful for vascular tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 5939-5946 |
| Number of pages | 8 |
| Journal | Biomaterials |
| Volume | 25 |
| Issue number | 28 |
| DOIs | |
| Publication status | Published - 2004 Dec 1 |
| Externally published | Yes |
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Keywords
- Biodegradation
- Compatibility to smooth muscle cells
- Elastic PLCL scaffolds
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Biomedical Engineering
Cite this
In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds. / Jeong, Sung In; Kim, Byung Soo; Kang, Sun Woong; Kwon, Jae Hyun; Lee, Young Moo; Kim, Soo Hyun; Kim, Young Ha.
In: Biomaterials, Vol. 25, No. 28, 01.12.2004, p. 5939-5946.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds
AU - Jeong, Sung In
AU - Kim, Byung Soo
AU - Kang, Sun Woong
AU - Kwon, Jae Hyun
AU - Lee, Young Moo
AU - Kim, Soo Hyun
AU - Kim, Young Ha
PY - 2004/12/1
Y1 - 2004/12/1
N2 - Tubular scaffolds were fabricated from very elastic poly(L-lactide-co- ε-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, masson trichrome staining, SM α-actin antibody, and CM-DiI labeling confirmed that the regular morphology and biofunction of the SMCs seeded and the expression of the vascular smooth muscle matrices in PLCL scaffolds. The implanted PLCL scaffolds displayed a slow degradation on time, where caprolactone units were faster degraded than lactide did. This could be explained by the fact that amorphous regions composed of mainly CL moieties degraded earlier than hard domains where most of the LA units were located. From these results, the scaffolds applied in this study were found to exhibit excellent tissue compatibility to SMCs and might be very useful for vascular tissue engineering.
AB - Tubular scaffolds were fabricated from very elastic poly(L-lactide-co- ε-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, masson trichrome staining, SM α-actin antibody, and CM-DiI labeling confirmed that the regular morphology and biofunction of the SMCs seeded and the expression of the vascular smooth muscle matrices in PLCL scaffolds. The implanted PLCL scaffolds displayed a slow degradation on time, where caprolactone units were faster degraded than lactide did. This could be explained by the fact that amorphous regions composed of mainly CL moieties degraded earlier than hard domains where most of the LA units were located. From these results, the scaffolds applied in this study were found to exhibit excellent tissue compatibility to SMCs and might be very useful for vascular tissue engineering.
KW - Biodegradation
KW - Compatibility to smooth muscle cells
KW - Elastic PLCL scaffolds
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UR - http://www.scopus.com/inward/citedby.url?scp=2942607678&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2004.01.057
DO - 10.1016/j.biomaterials.2004.01.057
M3 - Article
C2 - 15183608
AN - SCOPUS:2942607678
VL - 25
SP - 5939
EP - 5946
JO - Biomaterials
JF - Biomaterials
SN - 0142-9612
IS - 28
ER -