In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds

Sung In Jeong, Byung Soo Kim, Sun Woong Kang, Jae Hyun Kwon, Young Moo Lee, Soo Hyun Kim, Young Ha Kim

Research output: Contribution to journalArticle

176 Citations (Scopus)

Abstract

Tubular scaffolds were fabricated from very elastic poly(L-lactide-co- ε-caprolactone) (PLCL, 50:50). The scaffolds were seeded with smooth muscle cells (SMCs) and implanted in nude mice to investigate the tissue compatibility and in vivo degradation behavior. Histological examination of all the implants with haematoxylin and eosin staining, masson trichrome staining, SM α-actin antibody, and CM-DiI labeling confirmed that the regular morphology and biofunction of the SMCs seeded and the expression of the vascular smooth muscle matrices in PLCL scaffolds. The implanted PLCL scaffolds displayed a slow degradation on time, where caprolactone units were faster degraded than lactide did. This could be explained by the fact that amorphous regions composed of mainly CL moieties degraded earlier than hard domains where most of the LA units were located. From these results, the scaffolds applied in this study were found to exhibit excellent tissue compatibility to SMCs and might be very useful for vascular tissue engineering.

Original languageEnglish
Pages (from-to)5939-5946
Number of pages8
JournalBiomaterials
Volume25
Issue number28
DOIs
Publication statusPublished - 2004 Dec 1
Externally publishedYes

Fingerprint

Scaffolds
Smooth Muscle Myocytes
Histocompatibility
Muscle
Degradation
Staining and Labeling
Hematoxylin
Tissue Engineering
Eosine Yellowish-(YS)
Vascular Smooth Muscle
Nude Mice
Blood Vessels
Actins
Tissue
Tissue engineering
Antibodies
Labeling
Cells
poly(lactide)
caprolactone

Keywords

  • Biodegradation
  • Compatibility to smooth muscle cells
  • Elastic PLCL scaffolds

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Jeong, S. I., Kim, B. S., Kang, S. W., Kwon, J. H., Lee, Y. M., Kim, S. H., & Kim, Y. H. (2004). In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds. Biomaterials, 25(28), 5939-5946. https://doi.org/10.1016/j.biomaterials.2004.01.057

In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds. / Jeong, Sung In; Kim, Byung Soo; Kang, Sun Woong; Kwon, Jae Hyun; Lee, Young Moo; Kim, Soo Hyun; Kim, Young Ha.

In: Biomaterials, Vol. 25, No. 28, 01.12.2004, p. 5939-5946.

Research output: Contribution to journalArticle

Jeong, SI, Kim, BS, Kang, SW, Kwon, JH, Lee, YM, Kim, SH & Kim, YH 2004, 'In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds', Biomaterials, vol. 25, no. 28, pp. 5939-5946. https://doi.org/10.1016/j.biomaterials.2004.01.057
Jeong, Sung In ; Kim, Byung Soo ; Kang, Sun Woong ; Kwon, Jae Hyun ; Lee, Young Moo ; Kim, Soo Hyun ; Kim, Young Ha. / In vivo biocompatibilty and degradation behavior of elastic poly(L-lactide-co-ε-caprolactone) scaffolds. In: Biomaterials. 2004 ; Vol. 25, No. 28. pp. 5939-5946.
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