In Vivo Characterization of Phosphotransferase-Encoding Genes istP and forP as Interchangeable Launchers of the C3',4'-Dideoxygenation Biosynthetic Pathway of 1,4-Diaminocyclitol Antibiotics

Nguyen Huong, Na Joon Lee, Hyun Ha Hwang, Hye Bin Son, Hye Ji Kim, Eun Gyo Seo, Nguyen Hoang, Je Won Park

Research output: Contribution to journalArticle

Abstract

Deactivation of aminoglycosides by their modifying enzymes, including a number of aminoglycoside O-phosphotransferases, is the most ubiquitous resistance mechanism in aminoglycoside-resistant pathogens. Nonetheless, in a couple of biosynthetic pathways for gentamicins, fortimicins, and istamycins, phosphorylation of aminoglycosides seems to be a unique and initial step for the creation of a natural defensive structural feature such as a 3',4'- dideoxy scaffold. Our aim was to elucidate the biochemical details on the beginning of these C3',4'-dideoxygenation biosynthetic steps for aminoglycosides. The biosynthesis of istamycins must surely involve these 3',4'-didehydroxylation steps, but much less has been reported in terms of characterization of istamycin biosynthetic genes, especially about the phosphotransferase-encoding gene. In the disruption and complementation experiments pointing to a putative gene, istP, in the genome of wild-type Streptomyces tenjimariensis, the function of the istP gene was proved here to be a phosphotransferase. Next, an in-frame deletion of a known phosphotransferase-encoding gene forP from the genome of wild-type Micromonospora olivasterospora resulted in the appearance of a hitherto unidentified fortimicin shunt product, namely 3-O-methyl-FOR-KK1, whereas complementation of forP restored the natural fortimicin metabolite profiles. The bilateral complementation of an istP gene (or forP) in the ΔforP mutant ( or ΔistP mutant strain) successfully restored the biosynthesis of 3',4'- dideoxy fortimicins and istamycins , thus clearly indicating that they are interchangeable launchers of the biosynthesis of 3',4'-dideoxy types of 1,4-diaminocyclitol antibiotics.

Original languageEnglish
Pages (from-to)367-372
Number of pages6
JournalJournal of microbiology and biotechnology
Volume29
Issue number3
DOIs
Publication statusPublished - 2019 Mar 28

Fingerprint

Biosynthetic Pathways
Phosphotransferases
Aminoglycosides
Anti-Bacterial Agents
Genes
Micromonospora
Kanamycin Kinase
Genome
Streptomyces
Gentamicins
Phosphorylation
istamycins
fortimicins
Enzymes

Keywords

  • 1-O-methyl-FOR-KK1
  • Aminoglycoside
  • fortimicin
  • istamycin
  • istP

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

In Vivo Characterization of Phosphotransferase-Encoding Genes istP and forP as Interchangeable Launchers of the C3',4'-Dideoxygenation Biosynthetic Pathway of 1,4-Diaminocyclitol Antibiotics. / Huong, Nguyen; Lee, Na Joon; Hwang, Hyun Ha; Son, Hye Bin; Kim, Hye Ji; Seo, Eun Gyo; Hoang, Nguyen; Park, Je Won.

In: Journal of microbiology and biotechnology, Vol. 29, No. 3, 28.03.2019, p. 367-372.

Research output: Contribution to journalArticle

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AU - Lee, Na Joon

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AU - Son, Hye Bin

AU - Kim, Hye Ji

AU - Seo, Eun Gyo

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