TY - JOUR
T1 - In vivo comparison of biomineralized scaffold-directed osteogenic differentiation of human embryonic and mesenchymal stem cells
AU - Wen, Cai
AU - Kang, Heemin
AU - Shih, Yu Ru V.
AU - Hwang, Yong Sung
AU - Varghese, Shyni
N1 - Funding Information:
This work was supported by National Institutes of Health (NIH; Grant 1 R01 AR063184-01A1). The hMSCs used in this study were provided by Texas A&M University from the NIH (Grant P40RR017447).
PY - 2016/4/1
Y1 - 2016/4/1
N2 - Human pluripotent stem cells such as embryonic stem cells (hESCs) and multipotent stem cells like mesenchymal stem cells (hMSCs) hold great promise as potential cell sources for bone tissue regeneration. Comparing the in vivo osteogenesis of hESCs and hMSCs by biomaterial-based cues provides insight into the differentiation kinetics of these cells as well as their potential to contribute to bone tissue repair in vivo. Here, we compared in vivo osteogenic differentiation of hESCs and hMSCs within osteoinductive calcium phosphate (CaP)-bearing biomineralized scaffolds that recapitulate a bone-specific mineral microenvironment. Both hESCs and hMSCs underwent osteogenic differentiation responding to the biomaterial-based instructive cues. Furthermore, hMSCs underwent earlier in vivo osteogenesis compared to hESCs, but both stem cell types acquired a similar osteogenic maturation by 8 weeks of implantation.
AB - Human pluripotent stem cells such as embryonic stem cells (hESCs) and multipotent stem cells like mesenchymal stem cells (hMSCs) hold great promise as potential cell sources for bone tissue regeneration. Comparing the in vivo osteogenesis of hESCs and hMSCs by biomaterial-based cues provides insight into the differentiation kinetics of these cells as well as their potential to contribute to bone tissue repair in vivo. Here, we compared in vivo osteogenic differentiation of hESCs and hMSCs within osteoinductive calcium phosphate (CaP)-bearing biomineralized scaffolds that recapitulate a bone-specific mineral microenvironment. Both hESCs and hMSCs underwent osteogenic differentiation responding to the biomaterial-based instructive cues. Furthermore, hMSCs underwent earlier in vivo osteogenesis compared to hESCs, but both stem cell types acquired a similar osteogenic maturation by 8 weeks of implantation.
KW - Biomineralized scaffold
KW - Bone tissue engineering
KW - Calcium phosphate
KW - Embryonic stem cells
KW - In vivo osteogenic differentiation
KW - Mesenchymal stem cells
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U2 - 10.1007/s13346-015-0242-2
DO - 10.1007/s13346-015-0242-2
M3 - Article
C2 - 26105532
AN - SCOPUS:84960091661
VL - 6
SP - 121
EP - 131
JO - Drug Delivery and Translational Research
JF - Drug Delivery and Translational Research
SN - 2190-393X
IS - 2
ER -