In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model

Seung Chul Rhee, Yi Hwa Ji, Nareg A. Gharibjanian, Eun-Sang Dhong, Seung Ha Park, Eul Sik Yoon

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33 Citations (Scopus)

Abstract

Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX®) and/or poly(70l-lactide-co-30dl-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM+SVF, (4) DBM+PLA, and (5) DBM+PLA+SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM+SVF and DBM+PLA+SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM+SVF: 57.69%; DBM+PLA: 24.86%; DBM+PLA+SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM+SVF and DBM+PLA+SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.

Original languageEnglish
Pages (from-to)233-242
Number of pages10
JournalStem Cells and Development
Volume20
Issue number2
DOIs
Publication statusPublished - 2011 Feb 1

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Bone Matrix
Bone Regeneration
Blood Vessels
Stem Cells
Bone and Bones
Osteogenesis
Pluripotent Stem Cells
Osteocalcin
Hematoxylin
Tissue Engineering
Eosine Yellowish-(YS)
Cell- and Tissue-Based Therapy
Adipose Tissue
Anti-Idiotypic Antibodies

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

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title = "In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model",
abstract = "Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX{\circledR}) and/or poly(70l-lactide-co-30dl-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM+SVF, (4) DBM+PLA, and (5) DBM+PLA+SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM+SVF and DBM+PLA+SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48{\%}; DBM: 39.94{\%}; DBM+SVF: 57.69{\%}; DBM+PLA: 24.86{\%}; DBM+PLA+SVF: 42.75{\%}). Histological evaluation revealed that there was abundant new bone formation in the DBM+SVF and DBM+PLA+SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.",
author = "Rhee, {Seung Chul} and Ji, {Yi Hwa} and Gharibjanian, {Nareg A.} and Eun-Sang Dhong and Park, {Seung Ha} and Yoon, {Eul Sik}",
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T1 - In vivo evaluation of mixtures of uncultured freshly isolated adipose-derived stem cells and demineralized bone matrix for bone regeneration in a rat critically sized calvarial defect model

AU - Rhee, Seung Chul

AU - Ji, Yi Hwa

AU - Gharibjanian, Nareg A.

AU - Dhong, Eun-Sang

AU - Park, Seung Ha

AU - Yoon, Eul Sik

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N2 - Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX®) and/or poly(70l-lactide-co-30dl-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM+SVF, (4) DBM+PLA, and (5) DBM+PLA+SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM+SVF and DBM+PLA+SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM+SVF: 57.69%; DBM+PLA: 24.86%; DBM+PLA+SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM+SVF and DBM+PLA+SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.

AB - Although many studies have suggested that human adipose tissue contains pluripotent stem cells, a few reports are available on stromal vascular fraction (SVF). In the present study, we evaluated the bone formation capacities of SVF. We implanted uncultured freshly isolated adipose-derived stem cells combined with demineralized bone matrix (DBM) to induce bone regeneration in a critically sized rat calvarial defect model. We used DBM (DBX®) and/or poly(70l-lactide-co-30dl-lactide) copolymer PLA as a scaffold. Fifty white rats were randomized to 5 different groups (n=10): (1) control, (2) DBM, (3) DBM+SVF, (4) DBM+PLA, and (5) DBM+PLA+SVF groups. After acquiring SVF, an 8-mm critically sized calvarial defect was made in each rat. Specimens were harvested at 8 weeks postimplantation and evaluated radiographically and histologically. New bone formation was qualified by hematoxylin and eosin staining and anti-osteocalcin antibody (OC4-30) immunostaining of calvarial sections. Amounts of mineralization were determined by radiodensitometric analysis. In gross appearance, the DBM+SVF and DBM+PLA+SVF groups showed more abundant bone formation than the other groups. Radiodensitometric evaluations revealed that significant intergroup differences were observed according to the Kruskal-Wallis (rank) test (P=0.030<0.05). The 5 groups show different amounts of filling of bone defects (control: 13.48%; DBM: 39.94%; DBM+SVF: 57.69%; DBM+PLA: 24.86%; DBM+PLA+SVF: 42.75%). Histological evaluation revealed that there was abundant new bone formation in the DBM+SVF and DBM+PLA+SVF groups. It was found that undifferentiated adipose-derived stem cells in the form of SVF induced new bone formation in rat calvarial defects. Accordingly, SVF offers a practical, promising candidate for regenerative tissue engineering or cell-based therapy.

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