In vivo imaging of endogenously produced HCLO in zebrafish and mice using a bright, photostable ratiometric fluorescent probe

Chong Duan, Miae Won, Peter Verwilst, Junchao Xu, Hyeong Seok Kim, Lintao Zeng, Jong Seung Kim

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60 Citations (Scopus)

Abstract

A high brightness red fluorescent probe (S-BODIPY) has been developed for the sensitive and specific imaging of HClO/ClO - in vitro and in vivo. This probe exhibits some distinctive features such as excellent resistance to photobleaching, a high fluorescence brightness, high selectivity, as well as a good biocompatibility. Upon oxidation of the thio-ether group into sulfoxide, the probe showed a noticeable ratiometric fluorescence response toward ClO - with fast response (within 30 s) and a low detection limit (59 nM). The probe demonstrated the successful imaging of exogenous and endogenous HClO/ClO - in living HeLa cells, zebrafish, and mice with high signal-to-noise ratios. S-BODIPY allows for the real-time monitoring the level of ClO - in living cells by ratiometric fluorescence imaging, opening up exciting prospects to develop red and even near-infrared BODIPYs with high brightness and good photostability for in vivo imaging.

Original languageEnglish
Pages (from-to)4172-4178
Number of pages7
JournalAnalytical Chemistry
Volume91
Issue number6
DOIs
Publication statusPublished - 2019 Mar 19

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ASJC Scopus subject areas

  • Analytical Chemistry

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