In vivo imaging of near-membrane calcium ions with two-photon probes

Chang Su Lim, Min Young Kang, Ji Hee Han, Isravel Antony Danish, Bong-Rae Cho

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We report a two-photon (TP) probe (ACaLN) for near-membrane Ca 2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca 2+, dissociation constants (K d TP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca 2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.

Original languageEnglish
Pages (from-to)2028-2033
Number of pages6
JournalChemistry - An Asian Journal
Volume6
Issue number8
DOIs
Publication statusPublished - 2011 Aug 1

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Photons
Ions
Calcium
Membranes
Imaging techniques
Metal ions
Fluorescence
Tissue

Keywords

  • calcium
  • fluorescence
  • microscopy
  • nonlinear spectroscopy
  • two-photon probe

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

In vivo imaging of near-membrane calcium ions with two-photon probes. / Lim, Chang Su; Kang, Min Young; Han, Ji Hee; Danish, Isravel Antony; Cho, Bong-Rae.

In: Chemistry - An Asian Journal, Vol. 6, No. 8, 01.08.2011, p. 2028-2033.

Research output: Contribution to journalArticle

Lim, Chang Su ; Kang, Min Young ; Han, Ji Hee ; Danish, Isravel Antony ; Cho, Bong-Rae. / In vivo imaging of near-membrane calcium ions with two-photon probes. In: Chemistry - An Asian Journal. 2011 ; Vol. 6, No. 8. pp. 2028-2033.
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