We report a two-photon (TP) probe (ACaLN) for near-membrane Ca2+ that shows a 13-fold TP excited fluorescence (TPEF) enhancement in response to Ca2+, dissociation constants (KdTP) of (1.9±0.2) μM, pH-insensitivity at the biologically relevant pH, and can detect near-membrane Ca2+ in live cells for more than 1500 s and in living tissues at 120 μm depth without interference from other metal ions. Comparison with existing probes provides a useful strategy for the design of efficient TP probes for the near-membrane metal ions.
|Number of pages||6|
|Journal||Chemistry - An Asian Journal|
|Publication status||Published - 2011 Aug 1|
- nonlinear spectroscopy
- two-photon probe
ASJC Scopus subject areas
- Organic Chemistry