In vivo near-infrared fluorescence imaging of apoptosis using histone H1-targeting peptide probe after anti-cancer treatment with cisplatin and cetuximab for early decision on tumor response

Hyun Kyung Jung, Kai Wang, Min Kyu Jung, In-San Kim, Byung Heon Lee

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Early decision on tumor response after anti-cancer treatment is still an unmet medical need. Here we investigated whether in vivo imaging of apoptosis using linear and cyclic (disulfide-bonded) form of ApoPep-1, a peptide that recognizes histone H1 exposed on apoptotic cells, at an early stage after treatment could predict tumor response to the treatment later. Treatment of stomach tumor cells with cistplatin or cetuximab alone induced apoptosis, while combination of cisplatin plus cetuximab more efficiently induced apoptosis, as detected by binding with linear and cyclic form of ApoPep-1. However, the differences between the single agent and combination treatment were more remarkable as detected with the cyclic form compared to the linear form. In tumor-bearing mice, apoptosis imaging was performed 1 week and 2 weeks after the initiation of treatment, while tumor volumes and weights were measured 3 weeks after the treatment. In vivo fluorescence imaging signals obtained by the uptake of ApoPep-1 to tumor was most remarkable in the group injected with cyclic form of ApoPep-1 at 1 week after combined treatment with cisplatin plus cetuximab. Correlation analysis revealed that imaging signals by cyclic ApoPep-1 at 1 week after treatment with cisplatin plus cetuximab in combination were most closely related with tumor volume changes (r2 = 0.934). These results demonstrate that in vivo apoptosis imaging using Apopep-1, especially cyclic ApoPep-1, is a sensitive and predictive tool for early decision on stomach tumor response after anti-cancer treatment.

Original languageEnglish
Article numbere100341
JournalPloS one
Volume9
Issue number6
DOIs
Publication statusPublished - 2014 Jun 20
Externally publishedYes

Fingerprint

cisplatin
Oncology
Optical Imaging
histones
Histones
Cisplatin
Tumors
apoptosis
Fluorescence
image analysis
peptides
fluorescence
Apoptosis
Infrared radiation
Imaging techniques
Peptides
neoplasms
Neoplasms
Tumor Burden
stomach neoplasms

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

In vivo near-infrared fluorescence imaging of apoptosis using histone H1-targeting peptide probe after anti-cancer treatment with cisplatin and cetuximab for early decision on tumor response. / Jung, Hyun Kyung; Wang, Kai; Jung, Min Kyu; Kim, In-San; Lee, Byung Heon.

In: PloS one, Vol. 9, No. 6, e100341, 20.06.2014.

Research output: Contribution to journalArticle

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