In vivo quantitative measurement of arthritis activity based on hydrophobically modified glycol chitosan in inflammatory arthritis

More active than passive accumulation

Kyeong Soon Park, Jin Hee Kang, Keum Hee Sa, Hee Beom Koo, Hyun Jung Cho, Eon Jeong Nam, In Chan Youn, Kwang Meyung Kim, In-San Kim, Ick Chan Kwon, Kui Won Choi, Young Mo Kang

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We demonstrated that arthritis could be visualized noninvasively using hydrophobically modified glycol chitosan nanoparticles labeled with Cy5.5 (HGC-Cy5.5) and an optical imaging system. Activated macrophages expressing Mac-1 molecules effectively phagocytosed HGC-Cy5.5, which formed spherical nanoparticles under physiologic conditions. We estimated the applicability of HGC-Cy5.5 to quantitative analysis of arthritis development and progression. Near-infrared fluorescence images, captured after HGCCy5.5 injection in mice with collagen-induced arthritis, showed stronger fluorescence intensity in the active arthritis group than in the nonarthritis group. According to the progression of arthritis in both collagen-induced arthritis and collagen antibody-induced arthritis models, total photon counts (TPCs) increased in parallel with the clinical arthritis index. Quantitative analysis of fluorescence after treatment with methotrexate showed a significant decrease in TPC in a dose-dependent manner. Histologic evaluation confirmed that the mechanism underlying selective accumulation of HGC-Cy5.5 within synovitis tissues included enhanced phagocytosis of the probe by Mac-1-expressing macrophages as well as enhanced permeability through leaky vessels. These results suggest that optical imaging of arthritis using HGC-Cy5.5 can provide an objective measurement of disease activity and, at the same time, therapeutic responses in rheumatoid arthritis.

Original languageEnglish
Pages (from-to)389-400
Number of pages12
JournalMolecular Imaging
Volume11
Issue number5
DOIs
Publication statusPublished - 2012 Sep 1
Externally publishedYes

Fingerprint

arthritis
Glycols
Chitosan
Collagen
Arthritis
glycols
Macrophages
Fluorescence
Experimental Arthritis
Photons
Nanoparticles
Optical Imaging
Chemical analysis
Phagocytosis
Antibodies
collagens
Imaging systems
macrophages
Tissue
Infrared radiation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biotechnology
  • Molecular Medicine
  • Biomedical Engineering
  • Condensed Matter Physics

Cite this

In vivo quantitative measurement of arthritis activity based on hydrophobically modified glycol chitosan in inflammatory arthritis : More active than passive accumulation. / Park, Kyeong Soon; Kang, Jin Hee; Sa, Keum Hee; Koo, Hee Beom; Cho, Hyun Jung; Nam, Eon Jeong; Youn, In Chan; Kim, Kwang Meyung; Kim, In-San; Kwon, Ick Chan; Choi, Kui Won; Kang, Young Mo.

In: Molecular Imaging, Vol. 11, No. 5, 01.09.2012, p. 389-400.

Research output: Contribution to journalArticle

Park, Kyeong Soon ; Kang, Jin Hee ; Sa, Keum Hee ; Koo, Hee Beom ; Cho, Hyun Jung ; Nam, Eon Jeong ; Youn, In Chan ; Kim, Kwang Meyung ; Kim, In-San ; Kwon, Ick Chan ; Choi, Kui Won ; Kang, Young Mo. / In vivo quantitative measurement of arthritis activity based on hydrophobically modified glycol chitosan in inflammatory arthritis : More active than passive accumulation. In: Molecular Imaging. 2012 ; Vol. 11, No. 5. pp. 389-400.
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