Inactivation of Escherichia coli O157: H7 on radish seeds by sequential treatments with chlorine dioxide, drying, and dry heat without loss of seed viability

Jihyun Bang, Haeyoung Kim, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon Ryu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We developed and validated a treatment to inactivate Escherichia coli O157:H7 on radish seeds without decreasing seed viability. Treatments with aqueous ClO 2 followed by drying and dry-heat treatments were evaluated for efficacy to inactivate the pathogen. Conditions to dry radish seeds after treatment with water (control) or ClO 2 were established. When treated seeds with high water activity (a w) (>0.99) were stored at 45°C and 23% relative humidity (RH), the a w decreased to <0.30 within 24 h. Drying high-a w seeds before exposing them to dry-heat treatment (≥60°C) was essential to preserve seed viability. The germination rate of radish seeds which had been immersed in water for 5 min, dried at 45°C and 23% RH for 24 h, and heated at 70°C for 48 h or at 80°C for 24 h was not significantly decreased (P ≤ 0.05) compared to that of untreatedradish seeds. Sequential treatments with ClO 2 (500 μg/ml, 5 min), drying (45°C, 23% RH, 24 h), and dry heating (70°C, 23% RH, 48 h) eliminated E. coli O157:H7 (5.9 log CFU/g) on radish seeds and, consequently, sprouts produced from them without decreasing the germination rate. These sequential treatments are recommended for application to radish seeds intended for sprout production.

Original languageEnglish
Pages (from-to)6680-6686
Number of pages7
JournalApplied and Environmental Microbiology
Volume77
Issue number18
DOIs
Publication statusPublished - 2011 Sep 1

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chlorine dioxide
Raphanus
Escherichia coli O157
radishes
chlorine
Seeds
inactivation
viability
Hot Temperature
drying
seed
heat
seeds
Humidity
relative humidity
sprouts (food)
Germination
germination
loss
heat treatment

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Inactivation of Escherichia coli O157 : H7 on radish seeds by sequential treatments with chlorine dioxide, drying, and dry heat without loss of seed viability. / Bang, Jihyun; Kim, Haeyoung; Kim, Hoikyung; Beuchat, Larry R.; Ryu, Jee-Hoon.

In: Applied and Environmental Microbiology, Vol. 77, No. 18, 01.09.2011, p. 6680-6686.

Research output: Contribution to journalArticle

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