TY - JOUR
T1 - Increase of multidrug efflux pump expression in fluoroquinoloneresistant Salmonella mutants induced by ciprofloxacin selective pressure
AU - Kang, Hyun Wol
AU - Woo, Gun Jo
N1 - Funding Information:
This work was supported by grants from the Brain Korea 21 (BK21, T1300291), National Antimicrobial Resistance Management Program ( 11092NARMP158 ) of the Ministry of Food and Drug Safety , and Rural Development Administration (RDA, PJ007585 ). We would also like to thank the Korea University Food Safety Hall and Institute of Food and Biomedicine Safety for allowing the use of their equipment and facilities.
PY - 2014
Y1 - 2014
N2 - Multidrug-resistant foodborne pathogens are a leading public health concern, as antimicrobial resistance can lead to therapeutic failure. In this study, a ciprofloxacin-susceptible Salmonella Istanbul (Sal10- FC-KU12) was isolated from chicken meat obtained from a market in Korea to induce ciprofloxacinresistant mutants (SalML, SalMM, and SalMH). Minimum inhibitory concentrations (MICs) of 12 antibiotics were measured in the presence or absence of an efflux pump inhibitor. Expression levels of efflux pumprelated genes (acrB, acrF, marA, ramA, rob, and soxS)were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Elevated MICs for the derived mutants were shown to result from the action of the efflux pump, with increased expression of marA, ramA, and acrB compared with the wild-type strain. The results of this study suggest that continued use of ciprofloxacin might induce the emergence of Salmonella mutants resistant not only to fluoroquinolones, but also to several other classes of antimicrobials.
AB - Multidrug-resistant foodborne pathogens are a leading public health concern, as antimicrobial resistance can lead to therapeutic failure. In this study, a ciprofloxacin-susceptible Salmonella Istanbul (Sal10- FC-KU12) was isolated from chicken meat obtained from a market in Korea to induce ciprofloxacinresistant mutants (SalML, SalMM, and SalMH). Minimum inhibitory concentrations (MICs) of 12 antibiotics were measured in the presence or absence of an efflux pump inhibitor. Expression levels of efflux pumprelated genes (acrB, acrF, marA, ramA, rob, and soxS)were determined using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Elevated MICs for the derived mutants were shown to result from the action of the efflux pump, with increased expression of marA, ramA, and acrB compared with the wild-type strain. The results of this study suggest that continued use of ciprofloxacin might induce the emergence of Salmonella mutants resistant not only to fluoroquinolones, but also to several other classes of antimicrobials.
KW - Antimicrobial resistance
KW - Efflux pump
KW - Fluoroquinolone
KW - Salmonella enterica
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U2 - 10.1016/j.rvsc.2014.07.019
DO - 10.1016/j.rvsc.2014.07.019
M3 - Article
C2 - 25128419
AN - SCOPUS:84925127357
VL - 97
SP - 182
EP - 186
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -
