Increased 2,3-butanediol production by changing codon usages in Escherichia coli

Seo Young Park, Borim Kim, Soojin Lee, Min-Kyu Oh, Jong In Won, Jinwon Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The natural microorganism Escherichia coli without modification is not suitable for the efficient production of 2,3-butanediol (2,3-BD) on an industrial scale because of its poor metabolic performance. Metabolic capacities of E. coli have been improved to produce 2,3-BD efficiently, the performance of which is possible for producing such a product. Codon optimization with the ribosome-binding site for the efficient production of target genes (budA and budC) was achieved by molecular engineering, which allowed the metabolic engineering to proceed to the next level. As a result, comparing the productivity in 26 H, where the amount of p18COR was 1.04 g/L and that of p18WTR was 0.41 g/L, represents an approximate 60.6% increase in the productivity of the p18WTR with codon optimization. In other words, p18COR was 2.54-fold greater than p18WTR in the production of 2,3-BD.

Original languageEnglish
Pages (from-to)535-540
Number of pages6
JournalBiotechnology and Applied Biochemistry
Volume61
Issue number5
DOIs
Publication statusPublished - 2014 Sep 1

Keywords

  • 2 3-butanediol
  • acetoin reductase
  • acetolactate decarboxylase
  • codon optimization
  • Escherichia coli
  • ribosome-binding site

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Medicine
  • Bioengineering
  • Process Chemistry and Technology
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Medicine(all)

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