Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components

Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, Se Hoon Choe, Seung Wook Kim, Chulhwan Park, Sung Ok Han

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.

Original languageEnglish
Pages (from-to)237-243
Number of pages7
JournalEnzyme and Microbial Technology
Volume51
Issue number4
DOIs
Publication statusPublished - 2012 Sep 10

Fingerprint

Acetyltransferases
Yeast
Glycerol
Saccharomyces cerevisiae
Ethanol
Yeasts
Biomass
Ergosterol
Trehalose
Osmotic Pressure
Fermentation
Yield stress
Membranes
Productivity
Growth

Keywords

  • Ethanol
  • Glycerol
  • Saccharomyces cerevisiae
  • SAGA complex
  • SPT
  • Stress tolerance

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components. / Yu, Kyung Ok; Jung, Ju; Ramzi, Ahmad Bazli; Choe, Se Hoon; Kim, Seung Wook; Park, Chulhwan; Han, Sung Ok.

In: Enzyme and Microbial Technology, Vol. 51, No. 4, 10.09.2012, p. 237-243.

Research output: Contribution to journalArticle

@article{845accd36cbd47dba6db6152e757e8b4,
title = "Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components",
abstract = "During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.",
keywords = "Ethanol, Glycerol, Saccharomyces cerevisiae, SAGA complex, SPT, Stress tolerance",
author = "Yu, {Kyung Ok} and Ju Jung and Ramzi, {Ahmad Bazli} and Choe, {Se Hoon} and Kim, {Seung Wook} and Chulhwan Park and Han, {Sung Ok}",
year = "2012",
month = "9",
day = "10",
doi = "10.1016/j.enzmictec.2012.07.003",
language = "English",
volume = "51",
pages = "237--243",
journal = "Enzyme and Microbial Technology",
issn = "0141-0229",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components

AU - Yu, Kyung Ok

AU - Jung, Ju

AU - Ramzi, Ahmad Bazli

AU - Choe, Se Hoon

AU - Kim, Seung Wook

AU - Park, Chulhwan

AU - Han, Sung Ok

PY - 2012/9/10

Y1 - 2012/9/10

N2 - During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.

AB - During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.

KW - Ethanol

KW - Glycerol

KW - Saccharomyces cerevisiae

KW - SAGA complex

KW - SPT

KW - Stress tolerance

UR - http://www.scopus.com/inward/record.url?scp=84864958428&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864958428&partnerID=8YFLogxK

U2 - 10.1016/j.enzmictec.2012.07.003

DO - 10.1016/j.enzmictec.2012.07.003

M3 - Article

C2 - 22883559

AN - SCOPUS:84864958428

VL - 51

SP - 237

EP - 243

JO - Enzyme and Microbial Technology

JF - Enzyme and Microbial Technology

SN - 0141-0229

IS - 4

ER -