Abstract
During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.
| Original language | English |
|---|---|
| Pages (from-to) | 237-243 |
| Number of pages | 7 |
| Journal | Enzyme and Microbial Technology |
| Volume | 51 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2012 Sep 10 |
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Keywords
- Ethanol
- Glycerol
- Saccharomyces cerevisiae
- SAGA complex
- SPT
- Stress tolerance
ASJC Scopus subject areas
- Biochemistry
- Biotechnology
- Applied Microbiology and Biotechnology
Cite this
Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components. / Yu, Kyung Ok; Jung, Ju; Ramzi, Ahmad Bazli; Choe, Se Hoon; Kim, Seung Wook; Park, Chulhwan; Han, Sung Ok.
In: Enzyme and Microbial Technology, Vol. 51, No. 4, 10.09.2012, p. 237-243.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased ethanol production from glycerol by Saccharomyces cerevisiae strains with enhanced stress tolerance from the overexpression of SAGA complex components
AU - Yu, Kyung Ok
AU - Jung, Ju
AU - Ramzi, Ahmad Bazli
AU - Choe, Se Hoon
AU - Kim, Seung Wook
AU - Park, Chulhwan
AU - Han, Sung Ok
PY - 2012/9/10
Y1 - 2012/9/10
N2 - During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.
AB - During the industrial production of ethanol using yeast, the cells are exposed to stresses that affect their growth and productivity; therefore, stress-tolerant yeast strains are highly desirable. To increase ethanol production from glycerol, a greater tolerance to osmotic and ethanol stress was engineered in yeast strains that were impaired in endogenous glycerol production by the overexpression of both SPT3 and SPT15, components of the SAGA (Spt-Ada-Gcn5-acetyltransferase) complex. The engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) formed significantly more biomass compared to the strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), and both engineered strains displayed increased biomass when compared to the control YPH499 fps1Δgpd2Δ (pESC-TRP) strain. The trehalose accumulation and ergosterol content of these strains were 2.3-fold and 1.6-fold higher, respectively, than the parent strains, suggesting that levels of cellular membrane components were correlated with the enhanced stress tolerance of the engineered strains. Consequently, the ethanol production of the engineered strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupSpt3.15Cas) was 1.8-fold more than that of strain YPH499. fps1Δgpd2Δ (p. GcyaDak, p. GupCas), with about 8.1. g/L ethanol produced. In conclusion, we successfully established that the co-expression of SPT3 and SPT15 that improved the fermentation performance of the engineered yeast strains which produced higher ethanol yields than stress-sensitive yeast strains.
KW - Ethanol
KW - Glycerol
KW - Saccharomyces cerevisiae
KW - SAGA complex
KW - SPT
KW - Stress tolerance
UR - http://www.scopus.com/inward/record.url?scp=84864958428&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864958428&partnerID=8YFLogxK
U2 - 10.1016/j.enzmictec.2012.07.003
DO - 10.1016/j.enzmictec.2012.07.003
M3 - Article
C2 - 22883559
AN - SCOPUS:84864958428
VL - 51
SP - 237
EP - 243
JO - Enzyme and Microbial Technology
JF - Enzyme and Microbial Technology
SN - 0141-0229
IS - 4
ER -