Increased expression of a disintegrin and metalloprotease 8 in allergic rhinitis

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6 Citations (Scopus)

Abstract

Background: A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Methods: Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining. Results: The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusion: ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis.

Original languageEnglish
JournalAmerican Journal of Rhinology and Allergy
Volume25
Issue number3
DOIs
Publication statusPublished - 2011 May 1

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Disintegrins
Metalloproteases
Nasal Mucosa
Turbinates
Proteins
Messenger RNA
Mucous Membrane
Reverse Transcription
Allergic Rhinitis
Pneumonia
Membrane Proteins
Peptide Hydrolases
Epithelium
Western Blotting
Staining and Labeling
Inflammation
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

Cite this

@article{896e24dac21947c8992922619fa6d38c,
title = "Increased expression of a disintegrin and metalloprotease 8 in allergic rhinitis",
abstract = "Background: A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Methods: Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining. Results: The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusion: ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis.",
author = "Park, {Il Ho} and Choi, {Seong Woong} and Hyuk Choi and Tae-Hoon Kim and Lee, {Seung Hoon} and Lee, {Sang Hag} and Lee, {Heung Man}",
year = "2011",
month = "5",
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doi = "10.2500/ajra.2011.25.3581",
language = "English",
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T1 - Increased expression of a disintegrin and metalloprotease 8 in allergic rhinitis

AU - Park, Il Ho

AU - Choi, Seong Woong

AU - Choi, Hyuk

AU - Kim, Tae-Hoon

AU - Lee, Seung Hoon

AU - Lee, Sang Hag

AU - Lee, Heung Man

PY - 2011/5/1

Y1 - 2011/5/1

N2 - Background: A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Methods: Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining. Results: The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusion: ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis.

AB - Background: A disintegrin and metalloproteases (ADAMs) constitute a family of cell surface proteins containing disintegrin and metalloprotease domain, which associate features of adhesion molecules and proteases. ADAM8 is a member of the ADAM family and has recently been implicated to have a role in allergic lung inflammation. Therefore, our aims were to determine the expression of ADAM8 mRNA and protein and to localize ADAM8 protein in the nasal mucosa of patients with allergic rhinitis and control subjects. Methods: Inferior turbinate mucosa samples were obtained from 20 patients with allergic rhinitis and 20 matched healthy normal controls. ADAM8 mRNA was extracted from the inferior turbinate mucosa, and then reverse transcription-polymerase chain reaction was performed. Western blot testing was used to analyze differences in the level of ADAM8 protein expression between patients with allergic rhinitis and normal controls, and the ADAM8 protein was localized with immunohistochemical staining. Results: The level of expression of ADAM8 mRNA and protein in the nasal mucosa was significantly increased in patients with allergic rhinitis compared with normal controls. ADAM8 protein was expressed in the epithelium, infiltrating inflammatory cells, and submucosal glands. Conclusion: ADAM8 is expressed in human nasal mucosa and is increased in patients with allergic rhinitis. These results suggest a possible contribution for ADAM8 in chronic inflammation of the nasal mucosa in patients with allergic rhinitis.

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