Increased expression of acidic mammalian chitinase and chitotriosidase in the nasal mucosa of patients with allergic rhinitis

Woo Sung Cho, Tae-Hoon Kim, Heung Man Lee, Seung Hoon Lee, Sung Ho Lee, Joon Hyuk Yoo, Yeon Soo Kim, Sang Hag Lee

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives/Hypothesis: Acidic mammalian chitinase (AMCase) has emerged as an important mediator of allergic asthma in both animal models and in humans. Recently, chitotriosidase has been suggested to play a role in innate immunity because of its phagocytic-specific expression. Thus, AMCase and chitotriosidase may play a role in the pathogenesis of allergic nasal mucosa. The expression and pattern of distribution of AMCase and chitotriosidase were, therefore, determined in normal and allergic nasal mucosa. Study Design: Controlled, prospective study. Methods: Normal inferior turbinate mucosa was obtained in patients who were admitted for augmentation rhinoplasty. Allergic turbinate mucosa was obtained from patients who had perennial allergic rhinitis during septo-turbinate surgery. Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa. Results: The expression of AMCase and chitotriosidase mRNAs and proteins analyzed by RT-PCR and Western blot were detected in all normal and allergic turbinate mucosa tested. The levels of expression of AMCase and chitotriosidase mRNAs and proteins were increased in allergic turbinate mucosa compared with normal turbinate mucosa. In both normal and allergic turbinate mucosa, AMCase and chitotriosidase were detected in the epithelium, inflammatory cells, and submucosal glands. The staining intensity for AMCase and chitotriosidase was stronger in allergic nasal mucosa than normal nasal mucosa. Conclusions: AMCase and chitotriosidase are constitutively expressed in normal turbinate mucosa, suggesting involvement in defense against chitin-containing pathogens. Upregulation of these chitinases in allergic condition suggests that they may play a role in the nasal allergic reaction like other inflammatory mediators in allergic rhinitis.

Original languageEnglish
Pages (from-to)870-875
Number of pages6
JournalLaryngoscope
Volume120
Issue number5
DOIs
Publication statusPublished - 2010 May 1

Fingerprint

Chitinases
Nasal Mucosa
Turbinates
Mucous Membrane
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
Rhinitis, Allergic, Perennial
Allergic Rhinitis
chitotriosidase
Rhinoplasty
Messenger RNA
Chitin
Nose
Innate Immunity
Hypersensitivity
Proteins
Up-Regulation
Asthma
Epithelium
Animal Models

Keywords

  • Acidic mammalian chitinase
  • Allergic nasal mucosa
  • Chitotriosidase

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Increased expression of acidic mammalian chitinase and chitotriosidase in the nasal mucosa of patients with allergic rhinitis. / Cho, Woo Sung; Kim, Tae-Hoon; Lee, Heung Man; Lee, Seung Hoon; Lee, Sung Ho; Yoo, Joon Hyuk; Kim, Yeon Soo; Lee, Sang Hag.

In: Laryngoscope, Vol. 120, No. 5, 01.05.2010, p. 870-875.

Research output: Contribution to journalArticle

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abstract = "Objectives/Hypothesis: Acidic mammalian chitinase (AMCase) has emerged as an important mediator of allergic asthma in both animal models and in humans. Recently, chitotriosidase has been suggested to play a role in innate immunity because of its phagocytic-specific expression. Thus, AMCase and chitotriosidase may play a role in the pathogenesis of allergic nasal mucosa. The expression and pattern of distribution of AMCase and chitotriosidase were, therefore, determined in normal and allergic nasal mucosa. Study Design: Controlled, prospective study. Methods: Normal inferior turbinate mucosa was obtained in patients who were admitted for augmentation rhinoplasty. Allergic turbinate mucosa was obtained from patients who had perennial allergic rhinitis during septo-turbinate surgery. Reverse transcriptase-polymerase chain reaction (RT-PCR), immunohistochemistry, and Western blotting were applied to the normal and allergic nasal mucosa. Results: The expression of AMCase and chitotriosidase mRNAs and proteins analyzed by RT-PCR and Western blot were detected in all normal and allergic turbinate mucosa tested. The levels of expression of AMCase and chitotriosidase mRNAs and proteins were increased in allergic turbinate mucosa compared with normal turbinate mucosa. In both normal and allergic turbinate mucosa, AMCase and chitotriosidase were detected in the epithelium, inflammatory cells, and submucosal glands. The staining intensity for AMCase and chitotriosidase was stronger in allergic nasal mucosa than normal nasal mucosa. Conclusions: AMCase and chitotriosidase are constitutively expressed in normal turbinate mucosa, suggesting involvement in defense against chitin-containing pathogens. Upregulation of these chitinases in allergic condition suggests that they may play a role in the nasal allergic reaction like other inflammatory mediators in allergic rhinitis.",
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AU - Lee, Sung Ho

AU - Yoo, Joon Hyuk

AU - Kim, Yeon Soo

AU - Lee, Sang Hag

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