Increased expression of angiogenin in nasal polyps

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Abstract

Background: The aim of this study was to determine the expression and distribution of angiogenin in nasal polyps and to evaluate the implication of such expression with respect to the development of nasal polyps. Methods: Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyp samples were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid rhinosinusitis. A semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed for angiogenin mRNA. Sections of nasal polyps were immunostained for localization of angiogenin and quantitatively analyzed using computer-based image analysis. Western blot analysis was performed. Results: Semiquantitative RT-PCR and Western blot analysis showed that angiogenin expression in nasal polyps was increased compared with that in normal nasal mucosa. Using immunohistochemical staining, moderate-to-high levels of angiogenin were mainly localized in the infiltrating inflammatory cells of nasal polyps and faint staining was found in normal nasal mucosa. Conclusion: These results suggest that angiogenin may play a role in the pathophysiology of nasal polyps.

Original languageEnglish
JournalAmerican Journal of Rhinology and Allergy
Volume25
Issue number1
DOIs
Publication statusPublished - 2011 Jan 1

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Nasal Polyps
Nasal Mucosa
Reverse Transcriptase Polymerase Chain Reaction
Western Blotting
Staining and Labeling
Rhinoplasty
Turbinates
angiogenin
Mucous Membrane
Messenger RNA

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Immunology and Allergy

Cite this

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title = "Increased expression of angiogenin in nasal polyps",
abstract = "Background: The aim of this study was to determine the expression and distribution of angiogenin in nasal polyps and to evaluate the implication of such expression with respect to the development of nasal polyps. Methods: Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyp samples were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid rhinosinusitis. A semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed for angiogenin mRNA. Sections of nasal polyps were immunostained for localization of angiogenin and quantitatively analyzed using computer-based image analysis. Western blot analysis was performed. Results: Semiquantitative RT-PCR and Western blot analysis showed that angiogenin expression in nasal polyps was increased compared with that in normal nasal mucosa. Using immunohistochemical staining, moderate-to-high levels of angiogenin were mainly localized in the infiltrating inflammatory cells of nasal polyps and faint staining was found in normal nasal mucosa. Conclusion: These results suggest that angiogenin may play a role in the pathophysiology of nasal polyps.",
author = "Hwang, {Kyu Sung} and Park, {Il Ho} and Hyuk Choi and Lee, {Seung Hoon} and Lee, {Sang Hag} and Lee, {Heung Man}",
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T1 - Increased expression of angiogenin in nasal polyps

AU - Hwang, Kyu Sung

AU - Park, Il Ho

AU - Choi, Hyuk

AU - Lee, Seung Hoon

AU - Lee, Sang Hag

AU - Lee, Heung Man

PY - 2011/1/1

Y1 - 2011/1/1

N2 - Background: The aim of this study was to determine the expression and distribution of angiogenin in nasal polyps and to evaluate the implication of such expression with respect to the development of nasal polyps. Methods: Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyp samples were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid rhinosinusitis. A semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed for angiogenin mRNA. Sections of nasal polyps were immunostained for localization of angiogenin and quantitatively analyzed using computer-based image analysis. Western blot analysis was performed. Results: Semiquantitative RT-PCR and Western blot analysis showed that angiogenin expression in nasal polyps was increased compared with that in normal nasal mucosa. Using immunohistochemical staining, moderate-to-high levels of angiogenin were mainly localized in the infiltrating inflammatory cells of nasal polyps and faint staining was found in normal nasal mucosa. Conclusion: These results suggest that angiogenin may play a role in the pathophysiology of nasal polyps.

AB - Background: The aim of this study was to determine the expression and distribution of angiogenin in nasal polyps and to evaluate the implication of such expression with respect to the development of nasal polyps. Methods: Normal inferior turbinate mucosa was obtained from 20 patients undergoing surgery for augmentation rhinoplasty. Nasal polyp samples were obtained from 20 patients undergoing endoscopic sinus surgery for chronic polypoid rhinosinusitis. A semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) was performed for angiogenin mRNA. Sections of nasal polyps were immunostained for localization of angiogenin and quantitatively analyzed using computer-based image analysis. Western blot analysis was performed. Results: Semiquantitative RT-PCR and Western blot analysis showed that angiogenin expression in nasal polyps was increased compared with that in normal nasal mucosa. Using immunohistochemical staining, moderate-to-high levels of angiogenin were mainly localized in the infiltrating inflammatory cells of nasal polyps and faint staining was found in normal nasal mucosa. Conclusion: These results suggest that angiogenin may play a role in the pathophysiology of nasal polyps.

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