Increased expression of cFLIPL in colonic adenocarcinoma

Byung Kyu Ryu, Min Goo Lee, Sung-Gil Chi, Youn Wha Kim, Jae Hoon Park

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

During tumour progression, cancer cells use diverse mechanisms to escape from apoptosis-inducing stimuli, which may include receptor internalization, inhibition of signal pathways, and regulation of specific sets of genes. Substantial numbers of colon cancer cells have been observed to express Fas/Fas ligand, but are resistant to Fas-mediated apoptosis, suggesting that colonic tumours might develop specific mechanisms to overcome Fas-mediated apoptosis. Recently, cellular FLICE-like inhibitory protein (cFLIP) has been identified as an endogenous inhibitor of Fas- or other receptor-mediated apoptosis and its altered high expression has a suspected association with tumour development or progression. In an effort to investigate the prevalence of cFLIPL alterations in colon carcinomas and their possible implications for the progression of colon cancers, cFLIPL expression was analysed in adenocarcinomas and adenomatous polyps of colon, with matched normal tissues, at RNA and protein levels, by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. cFLIPL transcripts- were constitutively expressed in colon cancers and expression levels were significantly higher in carcinomas than in normal tissues (p<0.05). Overexpression of cFLIPL protein was found exclusively in carcinoma cells in all matched sets analysed and approximately three-fold induction was detected in cancer cells (p<0.05). The expression of cFLIPL protein was not significantly altered in adenomatous polyps compared with normal tissues. Taken together, these results strongly suggest that abnormal overexpression of cFLIPL is a frequent event in colon carcinomas and might contribute to in vivo tumour transformation.

Original languageEnglish
Pages (from-to)15-19
Number of pages5
JournalJournal of Pathology
Volume194
Issue number1
DOIs
Publication statusPublished - 2001 May 12
Externally publishedYes

Fingerprint

Adenocarcinoma
Colonic Neoplasms
Apoptosis
Carcinoma
Adenomatous Polyps
Neoplasms
Colon
CASP8 and FADD-Like Apoptosis Regulating Protein
Proteins
Fas Ligand Protein
Reverse Transcription
Signal Transduction
Immunohistochemistry
RNA
Polymerase Chain Reaction
Genes

Keywords

  • Apoptosis
  • cFLIP
  • Colon carcinoma
  • Immunohistochemistry
  • Quantitative RT-PCR

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Increased expression of cFLIPL in colonic adenocarcinoma. / Ryu, Byung Kyu; Lee, Min Goo; Chi, Sung-Gil; Kim, Youn Wha; Park, Jae Hoon.

In: Journal of Pathology, Vol. 194, No. 1, 12.05.2001, p. 15-19.

Research output: Contribution to journalArticle

Ryu, Byung Kyu ; Lee, Min Goo ; Chi, Sung-Gil ; Kim, Youn Wha ; Park, Jae Hoon. / Increased expression of cFLIPL in colonic adenocarcinoma. In: Journal of Pathology. 2001 ; Vol. 194, No. 1. pp. 15-19.
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