TY - JOUR
T1 - Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer
AU - Ahn, Suzie E.
AU - Choi, Jin W.
AU - Rengaraj, Deivendran
AU - Seo, Hee W.
AU - Lim, Whasun
AU - Han, Jae Y.
AU - Song, Gwonhwa
N1 - Funding Information:
We thank Yong-Sang Song, MD, PhD and Jeong Mook Lim, DVM, PhD (WCU Biomodulation Major, Department of Agricultural Biotechnology, Seoul National University) for pathologic diagnosis of chicken ovarian cancer. This work was supported by grants from the Korea Science and Engineering Foundation (KOSEF) (R01-2007-000-20456-0) and the World Class University (WCU) program (R31-10056) through the National Research Foundation of Korea funded by the Ministry of Education, Science, and Technology. The authors are appreciative of Dr. Fuller W. Bazer (Texas A&M University, USA and Seoul National University, Korea) for manuscript preparation and helpful discussions.
PY - 2010/8/21
Y1 - 2010/8/21
N2 - Background: Cysteine cathepsins (CTSs) are involved in the degradation and remodeling of the extracellular matrix and are associated with cell transformation, differentiation, motility, and adhesion. These functions are also related to cancer cell invasion and metastasis. Chickens spontaneously develop epithelial ovarian cancer and are therefore a good animal model for human ovarian cancer. However, no studies have investigated the expression of CTSs in chickens with ovarian cancer.Methods: Cancerous (n = 5) and normal (n = 3) ovaries were collected from 2-to 3-year-old hens, and ovarian tissue samples were collected for study. Ovarian cancers were evaluated with hematoxylin and eosin staining. Reverse transcriptase and quantitative PCR analyses, in situ hybridization analysis were performed to examine the mRNA expression pattern of three CTSs in detail, and protein expression of CTSB was evaluated.Results: The CTSB, CTSC, and CTSS genes were highly expressed in cancerous chicken ovaries. Messenger RNAs for the three CTSs were localized to a nodule area, a major characteristic of cancerous ovaries, but the three CTSs showed no specific localization in normal ovaries. Immunoreactive CTSB protein was present in the nodule area of cancerous ovaries.Conclusion: Our results suggest that CTSB, CTSC, and CTSS have important functions in the development of epithelial ovarian cancer.
AB - Background: Cysteine cathepsins (CTSs) are involved in the degradation and remodeling of the extracellular matrix and are associated with cell transformation, differentiation, motility, and adhesion. These functions are also related to cancer cell invasion and metastasis. Chickens spontaneously develop epithelial ovarian cancer and are therefore a good animal model for human ovarian cancer. However, no studies have investigated the expression of CTSs in chickens with ovarian cancer.Methods: Cancerous (n = 5) and normal (n = 3) ovaries were collected from 2-to 3-year-old hens, and ovarian tissue samples were collected for study. Ovarian cancers were evaluated with hematoxylin and eosin staining. Reverse transcriptase and quantitative PCR analyses, in situ hybridization analysis were performed to examine the mRNA expression pattern of three CTSs in detail, and protein expression of CTSB was evaluated.Results: The CTSB, CTSC, and CTSS genes were highly expressed in cancerous chicken ovaries. Messenger RNAs for the three CTSs were localized to a nodule area, a major characteristic of cancerous ovaries, but the three CTSs showed no specific localization in normal ovaries. Immunoreactive CTSB protein was present in the nodule area of cancerous ovaries.Conclusion: Our results suggest that CTSB, CTSC, and CTSS have important functions in the development of epithelial ovarian cancer.
UR - http://www.scopus.com/inward/record.url?scp=77955752516&partnerID=8YFLogxK
U2 - 10.1186/1477-7827-8-100
DO - 10.1186/1477-7827-8-100
M3 - Article
C2 - 20727192
AN - SCOPUS:77955752516
SN - 1477-7827
VL - 8
JO - Reproductive Biology and Endocrinology
JF - Reproductive Biology and Endocrinology
M1 - 100
ER -
