Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer

Suzie E. Ahn, Jin W. Choi, Deivendran Rengaraj, Hee W. Seo, Whasun Lim, Jae Y. Han, Gwonhwa Song

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Background: Cysteine cathepsins (CTSs) are involved in the degradation and remodeling of the extracellular matrix and are associated with cell transformation, differentiation, motility, and adhesion. These functions are also related to cancer cell invasion and metastasis. Chickens spontaneously develop epithelial ovarian cancer and are therefore a good animal model for human ovarian cancer. However, no studies have investigated the expression of CTSs in chickens with ovarian cancer.Methods: Cancerous (n = 5) and normal (n = 3) ovaries were collected from 2-to 3-year-old hens, and ovarian tissue samples were collected for study. Ovarian cancers were evaluated with hematoxylin and eosin staining. Reverse transcriptase and quantitative PCR analyses, in situ hybridization analysis were performed to examine the mRNA expression pattern of three CTSs in detail, and protein expression of CTSB was evaluated.Results: The CTSB, CTSC, and CTSS genes were highly expressed in cancerous chicken ovaries. Messenger RNAs for the three CTSs were localized to a nodule area, a major characteristic of cancerous ovaries, but the three CTSs showed no specific localization in normal ovaries. Immunoreactive CTSB protein was present in the nodule area of cancerous ovaries.Conclusion: Our results suggest that CTSB, CTSC, and CTSS have important functions in the development of epithelial ovarian cancer.

Original languageEnglish
Article number100
JournalReproductive Biology and Endocrinology
Volume8
DOIs
Publication statusPublished - 2010 Aug 21
Externally publishedYes

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Cathepsins
Ovarian Neoplasms
Cysteine
Ovary
Chickens
Messenger RNA
Hematoxylin
Eosine Yellowish-(YS)
Reverse Transcriptase Polymerase Chain Reaction
In Situ Hybridization
Extracellular Matrix
Cell Differentiation
Proteins
Animal Models
Staining and Labeling
Neoplasm Metastasis
Genes
Neoplasms

ASJC Scopus subject areas

  • Developmental Biology
  • Endocrinology
  • Reproductive Medicine
  • Medicine(all)

Cite this

Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer. / Ahn, Suzie E.; Choi, Jin W.; Rengaraj, Deivendran; Seo, Hee W.; Lim, Whasun; Han, Jae Y.; Song, Gwonhwa.

In: Reproductive Biology and Endocrinology, Vol. 8, 100, 21.08.2010.

Research output: Contribution to journalArticle

Ahn, Suzie E. ; Choi, Jin W. ; Rengaraj, Deivendran ; Seo, Hee W. ; Lim, Whasun ; Han, Jae Y. ; Song, Gwonhwa. / Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer. In: Reproductive Biology and Endocrinology. 2010 ; Vol. 8.
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