Increased expression of glycoprotein 340 in the ethmoid sinus mucosa of patients with chronic sinusitis

Tae-Hoon Kim, Sang Hag Lee, Heung Man Lee, Hak Hyun Jung, Seung Hoon Lee, Woo Sung Cho, Young Gi Cinn, Hwan Choe, Moo Pil Kim, Ik One Yoo, Ho Yeon Hwang

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7 Citations (Scopus)

Abstract

Objectives: To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria. Design: Prospective study. Setting: Tertiary academic institution. Patients: Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery. Intervention: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed. Main Outcome Measures: The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed. Results: Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa. Conclusion: These results suggest that gp340 may play a constitutive role in nasal defense and may be upregulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.

Original languageEnglish
Pages (from-to)1111-1114
Number of pages4
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume133
Issue number11
DOIs
Publication statusPublished - 2007 Nov 1

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Ethmoid Sinus
Sinusitis
Mucous Membrane
Reverse Transcription
Polymerase Chain Reaction
Western Blotting
glycoprotein 340
Gram-Negative Bacteria
Nose
Glycoproteins
Outcome Assessment (Health Care)
Prospective Studies
Inflammation

ASJC Scopus subject areas

  • Otorhinolaryngology

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Increased expression of glycoprotein 340 in the ethmoid sinus mucosa of patients with chronic sinusitis. / Kim, Tae-Hoon; Lee, Sang Hag; Lee, Heung Man; Jung, Hak Hyun; Lee, Seung Hoon; Cho, Woo Sung; Cinn, Young Gi; Choe, Hwan; Kim, Moo Pil; Yoo, Ik One; Hwang, Ho Yeon.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 133, No. 11, 01.11.2007, p. 1111-1114.

Research output: Contribution to journalArticle

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abstract = "Objectives: To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria. Design: Prospective study. Setting: Tertiary academic institution. Patients: Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery. Intervention: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed. Main Outcome Measures: The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed. Results: Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa. Conclusion: These results suggest that gp340 may play a constitutive role in nasal defense and may be upregulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.",
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