Abstract
Objectives: To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria. Design: Prospective study. Setting: Tertiary academic institution. Patients: Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery. Intervention: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed. Main Outcome Measures: The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed. Results: Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa. Conclusion: These results suggest that gp340 may play a constitutive role in nasal defense and may be upregulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.
| Original language | English |
|---|---|
| Pages (from-to) | 1111-1114 |
| Number of pages | 4 |
| Journal | Archives of Otolaryngology - Head and Neck Surgery |
| Volume | 133 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 2007 Nov 1 |
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ASJC Scopus subject areas
- Otorhinolaryngology
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Increased expression of glycoprotein 340 in the ethmoid sinus mucosa of patients with chronic sinusitis. / Kim, Tae-Hoon; Lee, Sang Hag; Lee, Heung Man; Jung, Hak Hyun; Lee, Seung Hoon; Cho, Woo Sung; Cinn, Young Gi; Choe, Hwan; Kim, Moo Pil; Yoo, Ik One; Hwang, Ho Yeon.
In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 133, No. 11, 01.11.2007, p. 1111-1114.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Increased expression of glycoprotein 340 in the ethmoid sinus mucosa of patients with chronic sinusitis
AU - Kim, Tae-Hoon
AU - Lee, Sang Hag
AU - Lee, Heung Man
AU - Jung, Hak Hyun
AU - Lee, Seung Hoon
AU - Cho, Woo Sung
AU - Cinn, Young Gi
AU - Choe, Hwan
AU - Kim, Moo Pil
AU - Yoo, Ik One
AU - Hwang, Ho Yeon
PY - 2007/11/1
Y1 - 2007/11/1
N2 - Objectives: To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria. Design: Prospective study. Setting: Tertiary academic institution. Patients: Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery. Intervention: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed. Main Outcome Measures: The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed. Results: Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa. Conclusion: These results suggest that gp340 may play a constitutive role in nasal defense and may be upregulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.
AB - Objectives: To investigate the expression and distribution of glycoprotein 340 (gp340), a secretory glycoprotein, in normal human sinus mucosa and inflammatory sinus mucosa and evaluate the possible effects of gp340 on the development of chronic sinusitis. Glycoprotein 340 was identified as a key element in the innate host defense mechanism on many mucosal surfaces and is directly involved in defense functions aimed at clearing gram-positive and gram-negative bacteria. Design: Prospective study. Setting: Tertiary academic institution. Patients: Normal sinus mucosa was obtained from the ethmoid sinus mucosa of 8 patients with blowout fractures undergoing endoscopic reduction. Inflammatory sinus mucosa was taken from 25 patients with chronic polypoid sinusitis during endoscopic sinus surgery. Intervention: Semiquantitative reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemical analysis, and Western blotting were performed. Main Outcome Measures: The expression level and distributional pattern of gp340 in normal and inflammatory sinus mucosa were analyzed. Results: Transcripts of the gp340 gene were detected in all human sinus tissues analyzed by RT-PCR. Immunohistochemical analysis revealed that gp340 is mainly localized in submucosal gland of both normal and inflammatory sinus mucosa. Semiquantitative RT-PCR and Western blot analysis showed the increased expression levels of gp340 in the inflammatory sinus mucosa compared with the normal sinus mucosa. Conclusion: These results suggest that gp340 may play a constitutive role in nasal defense and may be upregulated in response to inflammation, participating in antimicrobial defense in chronic sinusitis.
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U2 - 10.1001/archotol.133.11.1111
DO - 10.1001/archotol.133.11.1111
M3 - Article
C2 - 18025314
AN - SCOPUS:36348948580
VL - 133
SP - 1111
EP - 1114
JO - JAMA Otolaryngology - Head and Neck Surgery
JF - JAMA Otolaryngology - Head and Neck Surgery
SN - 2168-6181
IS - 11
ER -