Expresión aumentada del factor inducible por hipoxia 1α y del factor de crecimiento del tejido conectivo acompañando a fibrosis en testículos de ratas con varicocele

Translated title of the contribution: Increased expression of hypoxia-inducible factor-1α and connective tissue growth factor accompanied by fibrosis in the rat testis of varicocele

J. S. Paick, K. Park, S. W. Kim, J. W. Park, Je-Jong Kim, M. S. Kim, Jae Young Park

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: We investigated hypoxia inducible factor-1α (HIF-1α), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1α and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1α mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1α staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1α and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1α and CTGF expression and it is accompanied by fibrotic change in the testis.

Original languageSpanish
Pages (from-to)282-288
Number of pages7
JournalActas Urologicas Espanolas
Volume36
Issue number5
DOIs
Publication statusPublished - 2012 May 1

Fingerprint

Connective Tissue Growth Factor
Hypoxia-Inducible Factor 1
Varicocele
Testis
Fibrosis
Staining and Labeling
Reverse Transcription
Polymerase Chain Reaction
Messenger RNA
Orchiectomy
Sprague Dawley Rats
Immunohistochemistry
Control Groups

Keywords

  • Alpha subunit
  • Connective tissue growth factor
  • Fibrosis
  • Hypoxia inducible factor 1
  • Varicocele

ASJC Scopus subject areas

  • Urology

Cite this

Expresión aumentada del factor inducible por hipoxia 1α y del factor de crecimiento del tejido conectivo acompañando a fibrosis en testículos de ratas con varicocele. / Paick, J. S.; Park, K.; Kim, S. W.; Park, J. W.; Kim, Je-Jong; Kim, M. S.; Park, Jae Young.

In: Actas Urologicas Espanolas, Vol. 36, No. 5, 01.05.2012, p. 282-288.

Research output: Contribution to journalArticle

@article{fc0e51c542f4467b9a22e002f1db3946,
title = "Expresi{\'o}n aumentada del factor inducible por hipoxia 1α y del factor de crecimiento del tejido conectivo acompa{\~n}ando a fibrosis en test{\'i}culos de ratas con varicocele",
abstract = "Objective: We investigated hypoxia inducible factor-1α (HIF-1α), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1α and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1α mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1α staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1α and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1α and CTGF expression and it is accompanied by fibrotic change in the testis.",
keywords = "Alpha subunit, Connective tissue growth factor, Fibrosis, Hypoxia inducible factor 1, Varicocele",
author = "Paick, {J. S.} and K. Park and Kim, {S. W.} and Park, {J. W.} and Je-Jong Kim and Kim, {M. S.} and Park, {Jae Young}",
year = "2012",
month = "5",
day = "1",
doi = "10.1016/j.acuro.2011.07.022",
language = "Spanish",
volume = "36",
pages = "282--288",
journal = "Actas Urologicas Espanolas",
issn = "0210-4806",
publisher = "Elsevier Limited",
number = "5",

}

TY - JOUR

T1 - Expresión aumentada del factor inducible por hipoxia 1α y del factor de crecimiento del tejido conectivo acompañando a fibrosis en testículos de ratas con varicocele

AU - Paick, J. S.

AU - Park, K.

AU - Kim, S. W.

AU - Park, J. W.

AU - Kim, Je-Jong

AU - Kim, M. S.

AU - Park, Jae Young

PY - 2012/5/1

Y1 - 2012/5/1

N2 - Objective: We investigated hypoxia inducible factor-1α (HIF-1α), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1α and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1α mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1α staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1α and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1α and CTGF expression and it is accompanied by fibrotic change in the testis.

AB - Objective: We investigated hypoxia inducible factor-1α (HIF-1α), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1α and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1α mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1α staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1α and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1α and CTGF expression and it is accompanied by fibrotic change in the testis.

KW - Alpha subunit

KW - Connective tissue growth factor

KW - Fibrosis

KW - Hypoxia inducible factor 1

KW - Varicocele

UR - http://www.scopus.com/inward/record.url?scp=84862776871&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84862776871&partnerID=8YFLogxK

U2 - 10.1016/j.acuro.2011.07.022

DO - 10.1016/j.acuro.2011.07.022

M3 - Article

C2 - 22301018

AN - SCOPUS:84862776871

VL - 36

SP - 282

EP - 288

JO - Actas Urologicas Espanolas

JF - Actas Urologicas Espanolas

SN - 0210-4806

IS - 5

ER -