Expresión aumentada del factor inducible por hipoxia 1α y del factor de crecimiento del tejido conectivo acompañando a fibrosis en testículos de ratas con varicocele

Translated title of the contribution: Increased expression of hypoxia-inducible factor-1α and connective tissue growth factor accompanied by fibrosis in the rat testis of varicocele

J. S. Paick, K. Park, S. W. Kim, J. W. Park, J. J. Kim, M. S. Kim, J. Y. Park

Research output: Contribution to journalArticle

10 Citations (Scopus)


Objective: We investigated hypoxia inducible factor-1α (HIF-1α), connective tissue growth factor (CTGF) expression and fibrosis in the testis of rats with surgically induced varicocele. Material and methods: A total of 47 adult male Sprague-Dawley rats were arranged in 3 groups, namely group 1 (varicocele operation 4 weeks ago, n = 10; sham operation 4 weeks ago, n = 5), group 2 (8 weeks, n = 11; n = 5), and group 3 (12 weeks, n = 11; n = 5). The rats in every group underwent bilateral orchiectomy 4, 8, and 12 weeks after the operations, respectively. HIF-1α and CTGF expression of both testes in group 3 were studied by real-time reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Fibrotic change was assessed by quantitative image analysis. Results: HIF-1α mRNA expression in testes tissues in varicocele operation and sham controls showed no significant differences in RT-PCR. However, CTGF mRNA expressions in left testes were found to be significantly different between varicocele operation and sham controls. HIF-1α staining was present in both testes of all specimens and CTGF staining was present in 10 left and 8 right testes of 11 specimens. However HIF-1α and CTGF staining were absent in control group. There were significant fibrotic changes of both testes in groups 2 and 3. There were significant differences in fibrotic change along the durations of surgical varicocele. Conclusions: This study reveals that experimental varicocele in the rat is associated with HIF-1α and CTGF expression and it is accompanied by fibrotic change in the testis.

Original languageSpanish
Pages (from-to)282-288
Number of pages7
JournalActas Urologicas Espanolas
Issue number5
Publication statusPublished - 2012 May 1



  • Alpha subunit
  • Connective tissue growth factor
  • Fibrosis
  • Hypoxia inducible factor 1
  • Varicocele

ASJC Scopus subject areas

  • Urology

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