Increased expression of pigment epithelium-derived factor in allergic rhinitis

Hee Joon Kang, Hyo Hyun Park, Sungwon Chae, Soon Jae Hwang, Sang Hag Lee, Seung Hoon Lee, Heung Man Lee

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objectives: To investigate the expression of messenger RNA (mRNA) of the gene for pigment epithelium-derived factor (PEDF) (OMIM *172860) and PEDF protein and to localize the PEDF protein in the nasal mucosa of patients with allergic rhinitis and of control subjects. Design: Investigation of PEDF mRNA and PEDF protein expression in the nasal mucosa using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining. Participants: We used inferior turbinate mucosal samples from 10 patients with allergic rhinitis and 10 matched healthy control subjects. Interventions: We extracted PEDF mRNA from the inferior turbinate mucosa samples and performed reverse transcription-polymerase chain reaction analysis. We used Western blotting to analyze differences in expression levels of PEDF protein between patients with allergic rhinitis and healthy controls, and the PEDF protein was localized immunohistochemically. Results: The expression levels of PEDF mRNA and PEDF protein in the nasal mucosa were significantly increased in patients with allergic rhinitis compared with those in nonallergic controls. The PEDF protein was expressed in the epithelium and submucosal glands. Conclusions: We found that PEDF protein is expressed in the human nasal mucosa, and its expression is increased in allergic rhinitis. These results suggest a possible contribution of PEDF to the chronic inflammation of the nasal mucosa in allergic rhinitis.

Original languageEnglish
Pages (from-to)1094-1098
Number of pages5
JournalArchives of Otolaryngology - Head and Neck Surgery
Volume134
Issue number10
DOIs
Publication statusPublished - 2008 Oct 1

Fingerprint

Nasal Mucosa
Proteins
Turbinates
Messenger RNA
pigment epithelium-derived factor
Allergic Rhinitis
Reverse Transcription
Western Blotting
Genetic Databases
Polymerase Chain Reaction
Healthy Volunteers
Mucous Membrane
Epithelium
Staining and Labeling
Inflammation
Genes

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Surgery

Cite this

Increased expression of pigment epithelium-derived factor in allergic rhinitis. / Kang, Hee Joon; Park, Hyo Hyun; Chae, Sungwon; Hwang, Soon Jae; Lee, Sang Hag; Lee, Seung Hoon; Lee, Heung Man.

In: Archives of Otolaryngology - Head and Neck Surgery, Vol. 134, No. 10, 01.10.2008, p. 1094-1098.

Research output: Contribution to journalArticle

@article{bcc6f43771174e2b83b2470ae1c752bf,
title = "Increased expression of pigment epithelium-derived factor in allergic rhinitis",
abstract = "Objectives: To investigate the expression of messenger RNA (mRNA) of the gene for pigment epithelium-derived factor (PEDF) (OMIM *172860) and PEDF protein and to localize the PEDF protein in the nasal mucosa of patients with allergic rhinitis and of control subjects. Design: Investigation of PEDF mRNA and PEDF protein expression in the nasal mucosa using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining. Participants: We used inferior turbinate mucosal samples from 10 patients with allergic rhinitis and 10 matched healthy control subjects. Interventions: We extracted PEDF mRNA from the inferior turbinate mucosa samples and performed reverse transcription-polymerase chain reaction analysis. We used Western blotting to analyze differences in expression levels of PEDF protein between patients with allergic rhinitis and healthy controls, and the PEDF protein was localized immunohistochemically. Results: The expression levels of PEDF mRNA and PEDF protein in the nasal mucosa were significantly increased in patients with allergic rhinitis compared with those in nonallergic controls. The PEDF protein was expressed in the epithelium and submucosal glands. Conclusions: We found that PEDF protein is expressed in the human nasal mucosa, and its expression is increased in allergic rhinitis. These results suggest a possible contribution of PEDF to the chronic inflammation of the nasal mucosa in allergic rhinitis.",
author = "Kang, {Hee Joon} and Park, {Hyo Hyun} and Sungwon Chae and Hwang, {Soon Jae} and Lee, {Sang Hag} and Lee, {Seung Hoon} and Lee, {Heung Man}",
year = "2008",
month = "10",
day = "1",
doi = "10.1001/archotol.134.10.1094",
language = "English",
volume = "134",
pages = "1094--1098",
journal = "JAMA Otolaryngology - Head and Neck Surgery",
issn = "2168-6181",
publisher = "American Medical Association",
number = "10",

}

TY - JOUR

T1 - Increased expression of pigment epithelium-derived factor in allergic rhinitis

AU - Kang, Hee Joon

AU - Park, Hyo Hyun

AU - Chae, Sungwon

AU - Hwang, Soon Jae

AU - Lee, Sang Hag

AU - Lee, Seung Hoon

AU - Lee, Heung Man

PY - 2008/10/1

Y1 - 2008/10/1

N2 - Objectives: To investigate the expression of messenger RNA (mRNA) of the gene for pigment epithelium-derived factor (PEDF) (OMIM *172860) and PEDF protein and to localize the PEDF protein in the nasal mucosa of patients with allergic rhinitis and of control subjects. Design: Investigation of PEDF mRNA and PEDF protein expression in the nasal mucosa using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining. Participants: We used inferior turbinate mucosal samples from 10 patients with allergic rhinitis and 10 matched healthy control subjects. Interventions: We extracted PEDF mRNA from the inferior turbinate mucosa samples and performed reverse transcription-polymerase chain reaction analysis. We used Western blotting to analyze differences in expression levels of PEDF protein between patients with allergic rhinitis and healthy controls, and the PEDF protein was localized immunohistochemically. Results: The expression levels of PEDF mRNA and PEDF protein in the nasal mucosa were significantly increased in patients with allergic rhinitis compared with those in nonallergic controls. The PEDF protein was expressed in the epithelium and submucosal glands. Conclusions: We found that PEDF protein is expressed in the human nasal mucosa, and its expression is increased in allergic rhinitis. These results suggest a possible contribution of PEDF to the chronic inflammation of the nasal mucosa in allergic rhinitis.

AB - Objectives: To investigate the expression of messenger RNA (mRNA) of the gene for pigment epithelium-derived factor (PEDF) (OMIM *172860) and PEDF protein and to localize the PEDF protein in the nasal mucosa of patients with allergic rhinitis and of control subjects. Design: Investigation of PEDF mRNA and PEDF protein expression in the nasal mucosa using reverse transcription-polymerase chain reaction, Western blotting, and immunohistochemical staining. Participants: We used inferior turbinate mucosal samples from 10 patients with allergic rhinitis and 10 matched healthy control subjects. Interventions: We extracted PEDF mRNA from the inferior turbinate mucosa samples and performed reverse transcription-polymerase chain reaction analysis. We used Western blotting to analyze differences in expression levels of PEDF protein between patients with allergic rhinitis and healthy controls, and the PEDF protein was localized immunohistochemically. Results: The expression levels of PEDF mRNA and PEDF protein in the nasal mucosa were significantly increased in patients with allergic rhinitis compared with those in nonallergic controls. The PEDF protein was expressed in the epithelium and submucosal glands. Conclusions: We found that PEDF protein is expressed in the human nasal mucosa, and its expression is increased in allergic rhinitis. These results suggest a possible contribution of PEDF to the chronic inflammation of the nasal mucosa in allergic rhinitis.

UR - http://www.scopus.com/inward/record.url?scp=54349123332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=54349123332&partnerID=8YFLogxK

U2 - 10.1001/archotol.134.10.1094

DO - 10.1001/archotol.134.10.1094

M3 - Article

VL - 134

SP - 1094

EP - 1098

JO - JAMA Otolaryngology - Head and Neck Surgery

JF - JAMA Otolaryngology - Head and Neck Surgery

SN - 2168-6181

IS - 10

ER -