Increased expression of the F1Fo ATP synthase in response to iron in heart mitochondria

Misun Kim, Jinsun Kim, Choong Ill Cheon, Ho Cho Dae, Hoon Park Jong, Il Kim Keun, Kyo Young Lee, Eunsook Song

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The objective of the present study was to identify mitochondrial components associated with the damage caused by iron to the rat heart. Decreased cell viability was assessed by increased presence of lactate dehydrogenase (LDH) in serum. To assess the functional integrity of mitochondria, Reactive Oxygen Species (ROS), the Respiratory Control Ratio (RCR), ATP and chelatable iron content were measured in the heart. Chelatable iron increased 15-fold in the mitochondria and ROS increased by 59%. Deterioration of mitochondrial function in the presence of iron was demonstrated by low RCR (46% decrease) and low ATP content (96% decrease). Using two dimensional gel electrophoresis (2DE), we identified alterations in 21 mitochondrial proteins triggered by iron overload. Significantly, expression of the α, β, and d subunits of F 1Fo ATP synthase increased along with the loss of ATP. This suggests that the F1Fo ATP synthase participates in iron metabolism.

Original languageEnglish
Pages (from-to)153-157
Number of pages5
JournalJournal of Biochemistry and Molecular Biology
Volume41
Issue number2
DOIs
Publication statusPublished - 2008 Feb
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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