Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer

Jiyoon Jung, Sanghoon Jeong, Hoiseon Jeong, Hwa Eun Oh, Jung Woo Choi, Eung Seok Lee, Young Sik Kim, Yoonjin Kwak, Woo Ho Kim, Ju Han Lee

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we aimed to investigate the molecular biomarkers that are pivotal for the development and progression of gastric cancer (GC). We analyzed clinical specimens using RNA sequencing to identify the target genes. We found that the expression of HOXC6 mRNA was upregulated with the progression of cancer, which was validated by quantitative real time PCR and RNA in-situ hybridization. To compare the protein expression of HOXC6, we evaluated GC and normal gastric tissue samples using western blot analysis and immunohistochemistry. We detected significantly higher levels of HOXC6 in the GC tissues than in the normal controls at both mRNA and protein levels. The expression levels of HOXC6 mRNA in patients with advanced gastric cancer (AGC) were significantly higher than those in patients with early gastric cancer (EGC). Kaplan-Meier curves showed that high expression of HOXC6 mRNA is significantly associated with poor clinical prognosis. Our findings suggest that HOXC6 mRNA may be a novel biomarker and can be potentially valuable in predicting the prognosis of GC patients. Especially, HOXC6 mRNA in-situ hybridization may be a diagnostic tool for predicting prognosis of individual GC patients.

Original languageEnglish
Article numbere0236811
JournalPloS one
Volume15
Issue number8 August
DOIs
Publication statusPublished - 2020 Aug

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Fingerprint Dive into the research topics of 'Increased HOXC6 mRNA expression is a novel biomarker of gastric cancer'. Together they form a unique fingerprint.

Cite this