Increased methylation of the cytosolic 20-kD protein is accompanied by liver regeneration in a hepatectomized rat

Soon Young Kwon, Sohee Kim, Kyounghwa Lee, Tae Jin Kim, Seung Hoon Lee, Kyung-Mi Lee, Gil-Hong Park

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Arginine methylation has been implicated in the signal transduction pathway leading to cell growth. Here we show that a regenerating rat liver following partial hepatectomy exhibited elevated methyltransferase activity as shown by increased methylation of a subset of endogenous proteins in vitro. The 20-kDa protein was shown to be a major cytosolic protein undergoing methylation in regenerating hepatocytes. Methylation of the 20-kDa protein peaked at 1 d following partial hepatectomy, which gradually declined to a basal level within the next 14 d. Likewise, methylation of exogenously added bulk histones followed the similar time kinetics as the 20-kDa protein, reflecting time-dependent changes in methyltransferase activity in regenerating hepatocytes. Presence of exogenously added bulk histone in the in vitro methylation assay resulted in dose-dependent inhibition of methylation of the 20-kDa protein. All the histone subtypes tested, histone 1, 2A, 2B, 3 or 4, were able to inhibit methylation of the 20-kDa protein while addition of cytochrome C, α-lactalbumin, carbonic anhydrase, bovine serum albumin, and γ globulin minimally affected methylation of the 20-kDa protein. Since methylation of the 20-kDa protein preceded proliferation of hepatocytes upon partial hepatectomy, it is tempting to speculate that the methylated 20-kDa protein by activated histone-specific methyltransferase may be involved in an early signal critical for liver regeneration.

Original languageEnglish
Pages (from-to)85-92
Number of pages8
JournalExperimental and Molecular Medicine
Volume36
Issue number1
Publication statusPublished - 2004 Feb 29

Fingerprint

Liver Regeneration
Methylation
Liver
Rats
Histones
Proteins
Methyltransferases
Hepatectomy
Hepatocytes
Carbonic Anhydrase II
Lactalbumin
Serum Globulins
Signal transduction
Carbonic Anhydrases
Cell growth
Cytochromes
Bovine Serum Albumin
Arginine
Assays
Signal Transduction

Keywords

  • Arginine N-methylation
  • Cytosolic 20-kDa protein
  • Histone
  • Regenerating rat liver

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Increased methylation of the cytosolic 20-kD protein is accompanied by liver regeneration in a hepatectomized rat. / Kwon, Soon Young; Kim, Sohee; Lee, Kyounghwa; Kim, Tae Jin; Lee, Seung Hoon; Lee, Kyung-Mi; Park, Gil-Hong.

In: Experimental and Molecular Medicine, Vol. 36, No. 1, 29.02.2004, p. 85-92.

Research output: Contribution to journalArticle

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