Increased mitochondrial-encoded gene transcription in immortal DF-1 cells

Hyunggee Kim, Seungkwon You, In Jeong Kim, James Farris, Linda K. Foster, Douglas N. Foster

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-1 cells, we have used differential display RT-PCR. Of the numerous genes analyzed, three mitochondrial-encoded genes (ATPase 8/6, 16S rRNA, and cytochrome b) were shown to express at higher levels in DF-1 cells compared to primary and other immortal CEF cells. The inhibition of mitochondrial translation by treatment with chloramphenicol markedly decreased ATP production and cell proliferation in DF-1 cells, while not affecting growth in either primary or other immortal CEF cells. This result suggests a correlation between rapid cell proliferation and the increased mitochondrial respiratory functions. We also determined that the increased transcription of mitochondrial-encoded genes in DF-1 cells is due to increased de novo transcript synthesis as shown by mitochondrial run-on assays, and not the result of either increased mitochondrial biogenesis or mitochondrial transcript half-lives. Together, the present studies suggest that the transcriptional activation of mitochondrial-encoded genes and the elevated respiratory function should be one of the characteristics of rapidly dividing immortal cells.

Original languageEnglish
Pages (from-to)339-347
Number of pages9
JournalExperimental Cell Research
Volume265
Issue number2
DOIs
Publication statusPublished - 2001 May 1
Externally publishedYes

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Mitochondrial Genes
Chickens
Embryonic Structures
Fibroblasts
Cell Proliferation
Cell Line
Cytochromes b
Organelle Biogenesis
Chloramphenicol
Transcriptional Activation
Genes
Adenosine Triphosphatases
Cell Culture Techniques
Adenosine Triphosphate
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cell Biology

Cite this

Increased mitochondrial-encoded gene transcription in immortal DF-1 cells. / Kim, Hyunggee; You, Seungkwon; Kim, In Jeong; Farris, James; Foster, Linda K.; Foster, Douglas N.

In: Experimental Cell Research, Vol. 265, No. 2, 01.05.2001, p. 339-347.

Research output: Contribution to journalArticle

Kim, Hyunggee ; You, Seungkwon ; Kim, In Jeong ; Farris, James ; Foster, Linda K. ; Foster, Douglas N. / Increased mitochondrial-encoded gene transcription in immortal DF-1 cells. In: Experimental Cell Research. 2001 ; Vol. 265, No. 2. pp. 339-347.
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