Increased neuronal proliferation in the dentate gyrus of aged rats following neural stem cell implantation

Dong-Hyuk Park, David J. Eve, Paul R. Sanberg, James Musso, Adam D. Bachstetter, Alan Wolfson, Andrea Schlunk, Marc Olivier Baradez, John D. Sinden, Carmelina Gemma

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

It is now well accepted that the brain is able to generate newborn neurons from a population of resident multipotential neural stem cells (NSCs) located in two discrete regions of the brain. The capacity for neurogenesis appears to diminish over the lifespan of an organism. Methods to potentiate the proliferation of new neuronal or glial cells within the central nervous system from resident NSCs could have therapeutic potential following an insult, such as stroke, or to replace lost cells as a result of a neurodegenerative disease. We implanted cells from a human NSC cell line, CTX0E03, originally derived from fetal cortical tissue directly into the ventricles of aged rats. CTX0E03 cells have angiogenic properties via secretion of growth factors, so we investigated if the implanted cells would stimulate proliferation of NSCs within the subgranular zone (SGZ) of the dentate gyrus. Bromodeoxyuridine staining demonstrated significantly increased proliferation in the SGZ. Absence of double labeling for human nuclear antigen suggested that the increased proliferation was from endogenous neural progenitor cells. The acute treatment also led to an increased number of immature neurons as demonstrated by immunohistochemical staining for the immature neuronal marker doublecortin. The data suggest that implants of exogenous NSCs may promote regeneration in aging organisms through stimulation of endogenous neurogenesis.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalStem Cells and Development
Volume19
Issue number2
DOIs
Publication statusPublished - 2010 Feb 1
Externally publishedYes

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Neural Stem Cells
Dentate Gyrus
Neurogenesis
Staining and Labeling
Neurons
Nuclear Antigens
Brain
Bromodeoxyuridine
Neuroglia
Neurodegenerative Diseases
Regeneration
Intercellular Signaling Peptides and Proteins
Fetus
Stem Cells
Central Nervous System
Stroke
Cell Line
Population

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Hematology

Cite this

Increased neuronal proliferation in the dentate gyrus of aged rats following neural stem cell implantation. / Park, Dong-Hyuk; Eve, David J.; Sanberg, Paul R.; Musso, James; Bachstetter, Adam D.; Wolfson, Alan; Schlunk, Andrea; Baradez, Marc Olivier; Sinden, John D.; Gemma, Carmelina.

In: Stem Cells and Development, Vol. 19, No. 2, 01.02.2010, p. 175-180.

Research output: Contribution to journalArticle

Park, D-H, Eve, DJ, Sanberg, PR, Musso, J, Bachstetter, AD, Wolfson, A, Schlunk, A, Baradez, MO, Sinden, JD & Gemma, C 2010, 'Increased neuronal proliferation in the dentate gyrus of aged rats following neural stem cell implantation', Stem Cells and Development, vol. 19, no. 2, pp. 175-180. https://doi.org/10.1089/scd.2009.0172
Park, Dong-Hyuk ; Eve, David J. ; Sanberg, Paul R. ; Musso, James ; Bachstetter, Adam D. ; Wolfson, Alan ; Schlunk, Andrea ; Baradez, Marc Olivier ; Sinden, John D. ; Gemma, Carmelina. / Increased neuronal proliferation in the dentate gyrus of aged rats following neural stem cell implantation. In: Stem Cells and Development. 2010 ; Vol. 19, No. 2. pp. 175-180.
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