Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

Sang Jun Sim, Kristi D. Snell, Byung Woo Kim, Cho Kyun Rha, Anthony J. Sinskey

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.

Original languageEnglish
Pages (from-to)2057-2061
Number of pages5
JournalBiotechnology Letters
Volume23
Issue number24
DOIs
Publication statusPublished - 2001 Dec 1

Fingerprint

Hydroxybutyrates
Isopropyl Thiogalactoside
Chain length
Escherichia coli
Molecular Weight
Molecular weight
Modulation
Binding sites
Operon
Ribosomes
Binding Sites

Keywords

  • Average molecular weight
  • PHA synthase
  • Polyhydroxybutyrate

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

Cite this

Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli. / Sim, Sang Jun; Snell, Kristi D.; Kim, Byung Woo; Rha, Cho Kyun; Sinskey, Anthony J.

In: Biotechnology Letters, Vol. 23, No. 24, 01.12.2001, p. 2057-2061.

Research output: Contribution to journalArticle

Sim, Sang Jun ; Snell, Kristi D. ; Kim, Byung Woo ; Rha, Cho Kyun ; Sinskey, Anthony J. / Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli. In: Biotechnology Letters. 2001 ; Vol. 23, No. 24. pp. 2057-2061.
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