Increased poly-β-hydroxybutyrate (PHB) chain length by the modulation of PHA synthase activity in recombinant Escherichia coli

Sang Jun Sim, Kristi D. Snell, Byung Woo Kim, Cho Kyun Rha, Anthony J. Sinskey

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13 Citations (Scopus)

Abstract

A second promoter (PI) was inserted to the PHA (poly-β-hydroxyalkanoate) operon (pSP2) of Esherichia coli DH5α with an optimal E. coli ribosome binding site and a trc strong promoter (pSJS1) to obtain poly-β-hydroxybutyrate (PHB) with long chain length. When the inducer, IPTG was added to the culture at 0.4 mM, the average molecular weight was 1.1 × 106 Da. However, an even greater increase of the PHB average molecular weight to 2.5 × 107 Da was observed without IPTG being added.

Original languageEnglish
Pages (from-to)2057-2061
Number of pages5
JournalBiotechnology Letters
Volume23
Issue number24
DOIs
Publication statusPublished - 2001 Dec 1

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Keywords

  • Average molecular weight
  • PHA synthase
  • Polyhydroxybutyrate

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Bioengineering

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