TY - JOUR
T1 - Increased production of S-adenosyl-L-methionine using recombinant Saccharomyces cerevisiae sake K6
AU - Choi, Eun Sil
AU - Park, Bu Soo
AU - Lee, Sang Woo
AU - Oh, Min Kyu
N1 - Funding Information:
This work was supported by a scholarship from Yang Young Foundation and by a Korea Science and Engineering Foundation (KOSEF) grant (No. R01-2006-000-10143-0).
PY - 2009/1
Y1 - 2009/1
N2 - S. cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic marker. In this study, UV mutagenesis was conducted with S. cerevisiae sake K6. With the method, a mutant of sake yeast with leucine auxotroph, K6-1, was isolated. The mutant showed comparable growth rate and SAM productivity with its wild type. Using the auxotroph as a genetic marker, a SAM synthase in S. cerevisiae, SAM2, was overexpressed in the mutant strain. This recombinant DNA technology successfully increased SAM productivity in sake yeast.
AB - S. cerevisiae sake K6 was the firstly isolated industrial strain to overproduce S-adenosyl-L-methionine (SAM). Although the strain has advantages over other strains, such as GRAS (generally recognized as safe) property, the S. cerevisiae K6 has not been further developed with DNA recombinant technology due to the lack of a proper genetic marker. In this study, UV mutagenesis was conducted with S. cerevisiae sake K6. With the method, a mutant of sake yeast with leucine auxotroph, K6-1, was isolated. The mutant showed comparable growth rate and SAM productivity with its wild type. Using the auxotroph as a genetic marker, a SAM synthase in S. cerevisiae, SAM2, was overexpressed in the mutant strain. This recombinant DNA technology successfully increased SAM productivity in sake yeast.
KW - Auxotrophic mutant
KW - Recombinant DNA
KW - S-adenosyl-L-methionine
KW - Saccharomyces cerevisiae sake
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U2 - 10.1007/s11814-009-0025-x
DO - 10.1007/s11814-009-0025-x
M3 - Article
AN - SCOPUS:59349108457
VL - 26
SP - 156
EP - 159
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
SN - 0256-1115
IS - 1
ER -