Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1

H. Moon, Seung Wook Kim, Jeewon Lee, S. K. Rhee, E. S. Choi, H. A. Kang, Ik Hwan Kim, S. I. Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Volume111
Issue number2
DOIs
Publication statusPublished - 2003 Nov 1

Fingerprint

Batch Cell Culture Techniques
Pichia
Glycerol
Methanol
Growth
Epidermal Growth Factor
Cell growth
Substrates
Productivity
Kinetics

Keywords

  • Exponential feeding
  • Fed-batch culture
  • Hansenula polymorpha
  • Human epidermal growth factor
  • Mixed feed

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

Cite this

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title = "Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1",
abstract = "As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.",
keywords = "Exponential feeding, Fed-batch culture, Hansenula polymorpha, Human epidermal growth factor, Mixed feed",
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T1 - Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1

AU - Moon, H.

AU - Kim, Seung Wook

AU - Lee, Jeewon

AU - Rhee, S. K.

AU - Choi, E. S.

AU - Kang, H. A.

AU - Kim, Ik Hwan

AU - Hong, S. I.

PY - 2003/11/1

Y1 - 2003/11/1

N2 - As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.

AB - As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.

KW - Exponential feeding

KW - Fed-batch culture

KW - Hansenula polymorpha

KW - Human epidermal growth factor

KW - Mixed feed

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