Independent Exponential Feeding of Glycerol and Methanol for Fed-Batch Culture of Recombinant Hansenula polymorpha DL-1

H. Moon, Seung Wook Kim, Jeewon Lee, S. K. Rhee, E. S. Choi, H. A. Kang, Ik Hwan Kim, S. I. Hong

Research output: Contribution to journalArticle

6 Citations (Scopus)


As a novel feeding strategy for optimizing human epidermal growth factor (hEGF) production with a recombinant Hansenula polymorpha DL-1 using the methanol oxidase (MOX) promoter in H. polymorpha DL-1, independent exponential feeding of two substrates was used. A simple kinetic model considering the cell growth on two substrates was established and used to calculate the respective feeding rates of glycerol and methanol. In the fed-batch culture with methanol-only feeding, the optimal set point of specific growth rate on methanol was found to be 0.10 h-1. When the fed-batch cultures were conducted by the independent feeding of glycerol and methanol, the actual specific growth rate on glycerol and methanol was slightly lower than the set point of specific growth rate. By the uncoupled feeding of glycerol and methanol the volumetric productivity of hEGF increased from 6.4 to 8.0 mg/(L·h), compared with methanol-only feeding.

Original languageEnglish
Pages (from-to)65-79
Number of pages15
JournalApplied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology
Issue number2
Publication statusPublished - 2003 Nov 1



  • Exponential feeding
  • Fed-batch culture
  • Hansenula polymorpha
  • Human epidermal growth factor
  • Mixed feed

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Bioengineering

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