Independent spatial waves of biochemical differentiation along the surface of chicken brain as revealed by the sequential expression of acetylcholinesterase

Paul G. Layer, Susanne Rommel, Heinrich Bülthoff, Roland Hengstenberg

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

AChE-positive cells suddenly amass in a superficial layer of the neuroepithelium; this layer finally covers, in a sheat-like manner, the entire surface of the embryonic chicken brain. This feature is functionally not understood; however, it appears shortly after the neurons become post-mitotic, and the lateral extensions of this layer can easily be traced using histochemistry on serial brain sections. The layer can therefore be exploited to delineate spatially the waves of onset of biochemical tissue differentiation. We have studied whole brains between stages 11 and 30 and provide the first complete spatial schemes of brain differentiation based on computer-reconstructed, two- and three-dimensional maps. The brain does not differentiate in one smooth coherent wave, but instead five separate primary AChE-activation zones are detected: the first originating at stage 11 ("rhombencephalic wave"), the second at the same time ("midbrain wave"), the third at stage 15 (""tectal wave"). A fourth zone develops later, at stage 18, from the bottom part of the telencephalon to the top. Retinal development also starts at stage 18. In a given area, it appears that AChE-development shortly precedes that of the formation of major fiber tracts. AChE might therefore represent a prerequisite for fiber growth and pathfinding.

Original languageEnglish
Pages (from-to)587-595
Number of pages9
JournalCell and Tissue Research
Volume251
Issue number3
DOIs
Publication statusPublished - 1988 Mar

Keywords

  • Cell proliferation
  • Chicken
  • Computer-guided reconstruction ("Histol"-program)
  • Fiber tracts
  • Neural tracts
  • Pattern analysis
  • Pattern formation
  • Spatiotemporal brain development

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

Fingerprint Dive into the research topics of 'Independent spatial waves of biochemical differentiation along the surface of chicken brain as revealed by the sequential expression of acetylcholinesterase'. Together they form a unique fingerprint.

  • Cite this