Indole inhibits bacterial quorum sensing signal transmission by interfering with quorum sensing regulator folding

Jisun Kim, Woojun Park

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Quorum sensing (QS)-dependent biofilm formation and motility were controlled by AqsR in Acinetobacter oleivorans DR1. QS-controlled phenotypes appeared to be inhibited by indole and the aqsR mutant had the same phenotypes. We demonstrated that the turnover rate of AqsR became more rapid without the N-acylhomoserine lactone (AHL) signal, and that indole could increase the expression of many protease and chaperone proteins. The addition of exogenous indole decreased the expression of two AqsR-targeted genes: AOLE_03905 (putative surface adhesion protein) and AOLE_11355 (L-asparaginase). The overexpression of AqsR in Escherichia coli was impossible with the indole treatment. Surprisingly, our [35S]methionine pulse-labelling data demonstrated that the stability and folding of AqsR protein decreased in the presence of indole without changing aqsR mRNA expression in E. coli. Interestingly, indole resulted in a loss of TraR-dependent traG expression in an Agrobacterium tumefaciens indicator strain. However, when indole was added after incubation with exogenous AHL, indole could not inhibit the TraR-dependent expression of the traG promoter. This indicated that AHL-bound TraR could be protective against indole, but TraR without AHL could not be active in the presence of indole. Here, we provided evidence for the first time showing that the indole effect on QScontrolled bacterial phenotypes is due to inhibited QS regulator folding and not a reduced QS signal.

Original languageEnglish
Pages (from-to)2616-2625
Number of pages10
JournalMicrobiology (United Kingdom)
Volume159
Issue numberPART 12
DOIs
Publication statusPublished - 2013 Dec 1

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Quorum Sensing
Lactones
Phenotype
indole
Escherichia coli
Asparaginase
Agrobacterium tumefaciens
Acinetobacter
Protein Folding
Biofilms
Methionine
Membrane Proteins
Peptide Hydrolases

ASJC Scopus subject areas

  • Microbiology

Cite this

Indole inhibits bacterial quorum sensing signal transmission by interfering with quorum sensing regulator folding. / Kim, Jisun; Park, Woojun.

In: Microbiology (United Kingdom), Vol. 159, No. PART 12, 01.12.2013, p. 2616-2625.

Research output: Contribution to journalArticle

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