Induced tyramine overproduction in transgenic rice plants expressing a rice tyrosine decarboxylase under the control of methanol-inducible rice tryptophan decarboxylase promoter

Sangkyu Park, Kyungjin Lee, Young Soon Kim, Youn Tae Chi, Jeong Sheop Shin, Kyoungwhan Back

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Tyramine, one of the various biogenic amines found in plants, is derived from the aromatic L-amino acid tyrosine through the catalytic reaction of tyrosine decarboxylase (TYDC). Tyramine overproduction by constitutive expression of TYDC in rice plants leads to stunted growth, but an increased number of tillers. To regulate tyramine production in rice plants, we expressed TYDC under the control of a methanol-inducible plant tryptophan decarboxylase (TDC) promoter and generated transgenic T2 homozygous rice plants. The transgenic rice plants showed normal growth phenotypes with slightly increased levels of tyramine in seeds relative to wild type. Upon treatment with 1% methanol, the transgenic rice leaves produced large amounts of tyramine, whereas no increase in tyramine production was observed in wild-type plants. The methanol-induced accumulation of tyramine in the transgenic rice leaves was inversely correlated with the tyrosine level. These data indicate that tyramine production in rice plants can be artificially controlled using the methanol-inducible TDC promoter, suggesting that this promoter could be used to selectively induce the expression of other proteins or metabolites in rice plants.

Original languageEnglish
Pages (from-to)205-210
Number of pages6
JournalBioprocess and Biosystems Engineering
Volume35
Issue number1-2
DOIs
Publication statusPublished - 2012 Jan 1

Fingerprint

Tyrosine Decarboxylase
Aromatic-L-Amino-Acid Decarboxylases
Tyramine
Genetically Modified Plants
Methanol
Metabolites
Tyrosine
Seed
Amino acids
Amines
Growth Disorders
Proteins
Aromatic Amino Acids
Biogenic Amines
Oryza
Seeds

Keywords

  • Methanol
  • Transgenic rice
  • Tryptophan decarboxylase promoter
  • Tyramine
  • Tyrosine decarboxylase

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Induced tyramine overproduction in transgenic rice plants expressing a rice tyrosine decarboxylase under the control of methanol-inducible rice tryptophan decarboxylase promoter. / Park, Sangkyu; Lee, Kyungjin; Kim, Young Soon; Chi, Youn Tae; Shin, Jeong Sheop; Back, Kyoungwhan.

In: Bioprocess and Biosystems Engineering, Vol. 35, No. 1-2, 01.01.2012, p. 205-210.

Research output: Contribution to journalArticle

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