Induction of adaptive response by low-dose radiation in RIF cells transfected with Hspb1 (Hsp25) or inducible Hspa (Hsp70)

Yoon Jin Lee, Gil-Hong Park, Hye Nyun Cho, Chul Koo Cho, Young Mee Park, Su Jae Lee, Yun Sil Lee

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

An adaptive response results in a reduced effect of a high challenging dose of a stressor after a smaller, inducing dose has been applied a few hours earlier. Radiation-induced fibrosarcoma (RIF) cells did not show an adaptive response, i.e. a reduced effect from a high challenging dose (2 Gy) of a radiation after a priming dose (1 cGy) had been applied 4 or 7 h earlier, but cells of a thermoresistant clone (TR) derived from RIF cells did. Since the expression of inducible Hspa (also known as Hsp70) and Hspb1 (also known as Hsp25) was different in these two cell lines, the role of inducible Hspa and Hspb1 in the adaptive response was examined. When RIF cells were transfected with inducible Hspa or Hspb1, both radioresistance measured by clonogenic assays and a reduction of apoptosis were detected. The adaptive response was also acquired by these two cell lines. The inducible Hspa transfectant showed a more pronounced adaptive response than the Hspb1 transfectant. Based on these results, it appears that inducible Hspa and Hspb1 are at least partly responsible for the induction of the adaptive response in these cells. Moreover, when inducible Hspa or Hspb1 was transfected into RIF cells, coregulation of the two genes was detected. Heat-shock factor (Hsf) was found to be at least partially responsible for the induction of the adaptive response in these cells.

Original languageEnglish
Pages (from-to)371-377
Number of pages7
JournalRadiation Research
Volume157
Issue number4
DOIs
Publication statusPublished - 2002 Jan 1
Externally publishedYes

Fingerprint

Fibrosarcoma
induction
Radiation
dosage
radiation
cells
cultured cells
priming
Radiation Dosage
Cell Line
apoptosis
genes
Shock
Clone Cells
Hot Temperature
shock
Apoptosis
heat
Genes

ASJC Scopus subject areas

  • Biophysics
  • Radiation
  • Radiology Nuclear Medicine and imaging

Cite this

Induction of adaptive response by low-dose radiation in RIF cells transfected with Hspb1 (Hsp25) or inducible Hspa (Hsp70). / Lee, Yoon Jin; Park, Gil-Hong; Cho, Hye Nyun; Cho, Chul Koo; Park, Young Mee; Lee, Su Jae; Lee, Yun Sil.

In: Radiation Research, Vol. 157, No. 4, 01.01.2002, p. 371-377.

Research output: Contribution to journalArticle

Lee, Yoon Jin ; Park, Gil-Hong ; Cho, Hye Nyun ; Cho, Chul Koo ; Park, Young Mee ; Lee, Su Jae ; Lee, Yun Sil. / Induction of adaptive response by low-dose radiation in RIF cells transfected with Hspb1 (Hsp25) or inducible Hspa (Hsp70). In: Radiation Research. 2002 ; Vol. 157, No. 4. pp. 371-377.
@article{d898a8483ce844f0aa4fa0ee7c0371f6,
title = "Induction of adaptive response by low-dose radiation in RIF cells transfected with Hspb1 (Hsp25) or inducible Hspa (Hsp70)",
abstract = "An adaptive response results in a reduced effect of a high challenging dose of a stressor after a smaller, inducing dose has been applied a few hours earlier. Radiation-induced fibrosarcoma (RIF) cells did not show an adaptive response, i.e. a reduced effect from a high challenging dose (2 Gy) of a radiation after a priming dose (1 cGy) had been applied 4 or 7 h earlier, but cells of a thermoresistant clone (TR) derived from RIF cells did. Since the expression of inducible Hspa (also known as Hsp70) and Hspb1 (also known as Hsp25) was different in these two cell lines, the role of inducible Hspa and Hspb1 in the adaptive response was examined. When RIF cells were transfected with inducible Hspa or Hspb1, both radioresistance measured by clonogenic assays and a reduction of apoptosis were detected. The adaptive response was also acquired by these two cell lines. The inducible Hspa transfectant showed a more pronounced adaptive response than the Hspb1 transfectant. Based on these results, it appears that inducible Hspa and Hspb1 are at least partly responsible for the induction of the adaptive response in these cells. Moreover, when inducible Hspa or Hspb1 was transfected into RIF cells, coregulation of the two genes was detected. Heat-shock factor (Hsf) was found to be at least partially responsible for the induction of the adaptive response in these cells.",
author = "Lee, {Yoon Jin} and Gil-Hong Park and Cho, {Hye Nyun} and Cho, {Chul Koo} and Park, {Young Mee} and Lee, {Su Jae} and Lee, {Yun Sil}",
year = "2002",
month = "1",
day = "1",
doi = "10.1667/0033-7587(2002)157[0371:IOARBL]2.0.CO;2",
language = "English",
volume = "157",
pages = "371--377",
journal = "Radiation Research",
issn = "0033-7587",
publisher = "Radiation Research Society",
number = "4",

}

TY - JOUR

T1 - Induction of adaptive response by low-dose radiation in RIF cells transfected with Hspb1 (Hsp25) or inducible Hspa (Hsp70)

AU - Lee, Yoon Jin

AU - Park, Gil-Hong

AU - Cho, Hye Nyun

AU - Cho, Chul Koo

AU - Park, Young Mee

AU - Lee, Su Jae

AU - Lee, Yun Sil

PY - 2002/1/1

Y1 - 2002/1/1

N2 - An adaptive response results in a reduced effect of a high challenging dose of a stressor after a smaller, inducing dose has been applied a few hours earlier. Radiation-induced fibrosarcoma (RIF) cells did not show an adaptive response, i.e. a reduced effect from a high challenging dose (2 Gy) of a radiation after a priming dose (1 cGy) had been applied 4 or 7 h earlier, but cells of a thermoresistant clone (TR) derived from RIF cells did. Since the expression of inducible Hspa (also known as Hsp70) and Hspb1 (also known as Hsp25) was different in these two cell lines, the role of inducible Hspa and Hspb1 in the adaptive response was examined. When RIF cells were transfected with inducible Hspa or Hspb1, both radioresistance measured by clonogenic assays and a reduction of apoptosis were detected. The adaptive response was also acquired by these two cell lines. The inducible Hspa transfectant showed a more pronounced adaptive response than the Hspb1 transfectant. Based on these results, it appears that inducible Hspa and Hspb1 are at least partly responsible for the induction of the adaptive response in these cells. Moreover, when inducible Hspa or Hspb1 was transfected into RIF cells, coregulation of the two genes was detected. Heat-shock factor (Hsf) was found to be at least partially responsible for the induction of the adaptive response in these cells.

AB - An adaptive response results in a reduced effect of a high challenging dose of a stressor after a smaller, inducing dose has been applied a few hours earlier. Radiation-induced fibrosarcoma (RIF) cells did not show an adaptive response, i.e. a reduced effect from a high challenging dose (2 Gy) of a radiation after a priming dose (1 cGy) had been applied 4 or 7 h earlier, but cells of a thermoresistant clone (TR) derived from RIF cells did. Since the expression of inducible Hspa (also known as Hsp70) and Hspb1 (also known as Hsp25) was different in these two cell lines, the role of inducible Hspa and Hspb1 in the adaptive response was examined. When RIF cells were transfected with inducible Hspa or Hspb1, both radioresistance measured by clonogenic assays and a reduction of apoptosis were detected. The adaptive response was also acquired by these two cell lines. The inducible Hspa transfectant showed a more pronounced adaptive response than the Hspb1 transfectant. Based on these results, it appears that inducible Hspa and Hspb1 are at least partly responsible for the induction of the adaptive response in these cells. Moreover, when inducible Hspa or Hspb1 was transfected into RIF cells, coregulation of the two genes was detected. Heat-shock factor (Hsf) was found to be at least partially responsible for the induction of the adaptive response in these cells.

UR - http://www.scopus.com/inward/record.url?scp=0036202392&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036202392&partnerID=8YFLogxK

U2 - 10.1667/0033-7587(2002)157[0371:IOARBL]2.0.CO;2

DO - 10.1667/0033-7587(2002)157[0371:IOARBL]2.0.CO;2

M3 - Article

C2 - 11893238

AN - SCOPUS:0036202392

VL - 157

SP - 371

EP - 377

JO - Radiation Research

JF - Radiation Research

SN - 0033-7587

IS - 4

ER -